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  5. IL-21R Protein, Mouse (HEK293, Fc)

IL-21R Protein, Mouse (HEK293, Fc)

Cat. No.: HY-P73889
COA Handling Instructions

The IL-21R protein is specifically designed for IL-21 and forms a heterodimeric complex with the common γ subunit, which is critical for signal transduction. This receptor complex promotes interaction with Janus kinase 1 (JAK1), initiating downstream signaling events in response to interleukin-21. IL-21R Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived IL-21R protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IL-21R Protein, Mouse (HEK293, Fc) is 217 a.a., with molecular weight of 63-75 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The IL-21R protein is specifically designed for IL-21 and forms a heterodimeric complex with the common γ subunit, which is critical for signal transduction. This receptor complex promotes interaction with Janus kinase 1 (JAK1), initiating downstream signaling events in response to interleukin-21. IL-21R Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived IL-21R protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IL-21R Protein, Mouse (HEK293, Fc) is 217 a.a., with molecular weight of 63-75 kDa.

Background

IL-21R Protein functions as a receptor specifically designed for interleukin-21, forming a heterodimeric complex with the common gamma subunit. This receptor complex, crucial for transducing signals initiated by interleukin-21, enables cellular responses to this cytokine. The IL-21R heterodimeric structure facilitates its interaction with Janus kinase 1 (JAK1), playing a pivotal role in initiating downstream signaling events in response to interleukin-21 binding. The association of IL-21R with the common gamma subunit and its interaction with JAK1 collectively contribute to the transduction of interleukin-21-mediated signals, thus influencing various cellular processes and immune responses.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-21, at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-21R protein. The ED50 for this effect is 25.10 ng/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-21, at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-21R protein. The ED50 for this effect is 25.10 ng/mL.
Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

Q9JHX3 (C20-P236)

Gene ID

60504  [NCBI]

Molecular Construction
N-term
IL-21R (C20-P236)
Accession # Q9JHX3
hFc
C-term
Synonyms
Interleukin-21 receptor; IL-21 receptor; IL-21R; CD360; Nilr
AA Sequence

CLDLTCYTDYLWTITCVLETRSPNPSILSLTWQDEYEELQDQETFCSLHRSGHNTTHIWYTCHMRLSQFLSDEVFIVNVTDQSGNNSQECGSFVLAESIKPAPPLNVTVAFSGRYDISWDSAYDEPSNYVLRGKLQYELQYRNLRDPYAVRPVTKLISVDSRNVSLLPEEFHKDSSYQLQVRAAPQPGTSFRGTWSEWSDPVIFQTQAGEPEAGWDP

Molecular Weight

63-75 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4 (Normally trehalose is added as protectant before lyophilization. ) or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-21R Protein, Mouse (HEK293, Fc)
Cat. No.:
HY-P73889
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