1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-2 family (γc-family cytokines)
  5. IL-2R alpha/CD25 Protein, Human (HEK293, Fc)

IL-2R alpha/CD25 Protein, Human (HEK293, Fc)

Cat. No.: HY-P7374
Handling Instructions

IL-2R alpha/CD25 Protein, Human (HEK293, Fc) is a subunit of interleukin-2 receptor, plays a critical role in regulating the IL-2-mediated responses by modulating the affinity of the IL-2R for the ligand.

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $210 In-stock
Estimated Time of Arrival: December 31
50 μg   Get quote  
100 μg   Get quote  

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IL-2R alpha/CD25 Protein, Human (HEK293, Fc) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

HEK293

Tag

C-hFc

Accession

P01589 (E22-C213)

Gene ID

3559

Synonyms
rHuIL-2R α/CD25, Fc Chimera; TAC antigen; IL-2-RA; IL-2 R alpha; IL-2Rα; IL2RA
AA Sequence

ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC

Molecular Weight

65-75 kDa

Biological Activity
5 µg/mL (100 µL/well) of immoblized recombinant human IL-2 can bind human CD25/IL-2Rα-Fc with a linear range of 1.2-11 ng/mL.
Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Background

IL-2 binds with high affinity to a receptor consisting of three subunits: IL-2Rα (CD25), IL-2Rβ (CD122) and γc (CD132). Alpha subunit plays a critical role in regulating the IL-2-mediated responses by modulating the affinity of the IL-2R for the ligand. Then, two β and γ subunits initiate the signal transduction process via cytoplasmic tails[1].

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
IL-2R alpha/CD25 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P7374
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