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  4. IL-2 Receptor CD25/IL-2R alpha
  5. IL-2R alpha
  6. IL-2R alpha/CD25 Protein, Human (HEK293, Fc)

IL-2R alpha/CD25 Protein, Human (HEK293, Fc)

Cat. No.: HY-P7374
COA Handling Instructions

IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions. IL-2R alpha/CD25 Protein, Human (HEK293, Fc) is a recombinant human IL-2R alpha protein with an hFc tag at the C-terminus and is expressed in HEK293 cells. It consists of 192 amino acids (E22-C213).

For research use only. We do not sell to patients.

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Description

IL-2R alpha (CD25) is an essential component of high-affinity IL-2 receptors. IL-2R alpha enhances binding of IL-2 to its receptor complex so that regulates T cell growth and other lymphoid functions[1][2]. IL-2R alpha/CD25 Protein, Human (HEK293, Fc) is a recombinant human IL-2R alpha protein with an hFc tag at the C-terminus and is expressed in HEK293 cells. It consists of 192 amino acids (E22-C213).

Background

IL-2R alpha (CD25) is a type I membrane protein. IL-2R alpha is expressed in peripheral activated T and B cells, triple-negative thymocytes, and bone marrow pre-B cells. In high tumor regulatory T (Treg) cells, IL-2R alpha is highly expressed and is a potential target for Treg deletion. The expression of IL-2R alpha is undetectable on resting T cells[1][2][3].
The sequence of amino acids in IL-2R alpha from different species is very different (less than 85% similarity among human, rat and mouse).
IL-2R alpha is an essential component of high-affinity IL-2 receptors and has no signal-transducing activity per se. IL-2R alpha functions through enhancing binding of IL-2 to its receptor complex and acts as a positive feedback regulator. IL-2 is a principal growth factor for T lymphocytes and plays an important role in T cell immune response. IL-2R alpha transcription is regulated by three positive regulatory regions (PRRs): PRRI, PRRII and PRRIII. PRRIII is an IL-2 response element[1][2].
IL-2R alpha regulates T cell growth, augments lymphocyte activation and proliferation. IL-2R alpha is involved in preventing type 1 diabetes and cancers[1][2][4].

Biological Activity

1. 5 µg/mL (100 µL/well) of immoblized recombinant human IL-2 can bind human CD25/IL-2Rα-Fc with a linear range of 1.2-11 ng/mL.
2. Measured in a cell proliferation assay using Mo7e cells. The ED50 this effect is 0.1982 μg/mL in the presence of 30 ng/mL of recombinant human IL-2, corresponding to a specific activity is 5.05×103 units/mg.
3.Immobilized Human IL-2RA-Fc at 5 μg/mL (100 μl/well) can bind Human IL-2-His.The ED50 is 17.52 ng/mL.

  • Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 0.1982 μg/mL in the presence of 30 ng/mL of recombinant human IL-2, corresponding to a specific activity is 5.05×103 units/mg.
Species

Human

Source

HEK293

Tag

C-hFc

Accession

P01589 (E22-C213)

Gene ID
Molecular Construction
N-term
IL-2Rα (E22-C213)
Accession # P01589
hFc
C-term
Synonyms
rHuIL-2R α/CD25, Fc Chimera; TAC antigen; IL-2-RA; IL-2 R alpha; IL-2Rα; IL2RA
AA Sequence

ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC

Molecular Weight

Approximately 60-80 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-2R alpha/CD25 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P7374
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