1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors NK Cell CD Proteins
  4. IL-2 Receptor CD122/IL-2R beta
  5. IL-2R beta
  6. IL-2R beta/CD122 Protein, Canine (HEK293, Fc)

IL-2R beta/CD122 Protein, Canine (HEK293, Fc)

Cat. No.: HY-P76769
COA Handling Instructions

IL-2R beta (CD122), a type Ⅰ cytokine receptor expressed on T lymphocytes, is a receptor for IL-2 (Kd: 1 nM approximately). IL-2R beta mediates T cell immune responses, such as stimulating T cell proliferation and activating lymphokine-activated killer cells. IL-2R beta/CD122 Protein, Canine (HEK293, Fc) is a recombinant canine IL-2R beta (M1-P244) with a C-Terminal hFc tag, which is produced in HEK293 cells.

For research use only. We do not sell to patients.

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Description

IL-2R beta (CD122), a type Ⅰ cytokine receptor expressed on T lymphocytes, is a receptor for IL-2 (Kd: 1 nM approximately)[1]. IL-2R beta mediates T cell immune responses, such as stimulating T cell proliferation and activating lymphokine-activated killer cells[2]. IL-2R beta/CD122 Protein, Canine (HEK293, Fc) is a recombinant canine IL-2R beta (M1-P244) with a C-Terminal hFc tag, which is produced in HEK293 cells.

Background

IL-2R beta (CD122),一种表达于 T 淋巴细胞上的 Ⅰ 型细胞因子受体,是 IL-2 的受体。IL-2R beta 可介导 T 细胞免疫反应,如刺激 T 细胞增殖、激活淋巴因子激活的杀伤细胞。IL-2R beta/CD122 Protein, Canine (HEK293, Fc) 是一种由 HEK293 细胞产生的、重组犬 IL-2R beta (M1-P244), 其 C 末端带有 hFc 标签。

Biological Activity

Measured by its ability to inhibit the IL-15-dependent proliferation of Mo7e human megakaryocytic leukemic cells. The IC50 for this effect is 0.7659 μg/mL in the presence of 4 ng/mL of recombinant human IL-15, corresponding to a specific activity is 1.31×103 units/mg.

Species

Canine

Source

HEK293

Tag

C-hFc

Accession

NP_001273779 (S27-P245)

Gene ID

403439  [NCBI]

Molecular Construction
N-term
IL-2Rβ (S27-P245)
Accession # NP_001273779
hFc
C-term
Synonyms
Interleukin-2 receptor subunit beta; IL2RB; High affinity IL-2 receptor subunit beta; CD122
AA Sequence

SVVNDTSKLTCFYNSKANISCIWSWDGDLQATSCKIHAQPDRRPWNKSCELLPMRPAFWACYLILGSPDAQSLTSADVVGMSVMCHEGERWRILMTQDFKPFENLRLMAPNGLQVADVGTHKCNITWKVPQSSHYIKRYLEFEARKRSPGHSWEEASLMALRQNQQWISLETLTPDTLYEFQVRVRAQRGSHKTWSPWSQPLAFRTRPAARGKQSLPFP

Molecular Weight

Approximately 68-75 kDa due to the glycosylation

Purity

Greater than 80% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-2R beta/CD122 Protein, Canine (HEK293, Fc)
Cat. No.:
HY-P76769
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