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Irisin Protein, Human/Mouse/Rat (HEK293, His)

Cat. No.: HY-P70664
COA Handling Instructions

Irisin Protein, Human/Mouse/Rat (HEK293, His) is a Irisin protein labeled with a His-flag. Irisin is a hormone derived from the fibronectin type III domain-containing (FNDC5) gene that can promote energy expenditure via thermogenesis.

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Description

Irisin Protein, Human/Mouse/Rat (HEK293, His) is a Irisin protein labeled with a His-flag. Irisin is a hormone derived from the fibronectin type III domain-containing (FNDC5) gene that can promote energy expenditure via thermogenesis[1].

Background

Irisin is a recently identified hormone derived from the fibronectin type III domain-containing (FNDC5) gene that is released mainly from skeletal muscle after exercise or exposure to cold. Irisin is mainly secreted by muscle and is increased with exercise. In particular, Irisin has been shown to have beneficial effects in adipose tissues, brain and bone[2].
Recombinant Human/Mouse/Rat Irisin Protein (20-200 ng/ml; 24 hours) at low concentration has no effects on E-selectin expression. And when HUVECs are treated with high concentarion irisin (200 ng/ml), it results in soluble E-selectin concentrations similar to the levels detected in the control group treated with regular growth media[1].

Biological Activity

1.Measured by its ability to inhibit proliferation of A549 cells. The IC50 for this effect is 6.845 ng/mL, corresponding to a specific activity is 1.4609×105 units/mg.
2.Measured by its ability to induce p38 MAPK activation in 3T3 L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.

  • Measured by its ability to inhibit proliferation of A549 cells. The IC50 for this effect is 6.845 ng/mL , corresponding to a specific activity is 1.4609×105 units/mg.
Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q8NAU1 (D32-E143)

Gene ID
Molecular Construction
N-term
Irisin (D32-E143)
Accession # Q8NAU1
6*His
C-term
Synonyms
Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; Irisin; FNDC5
AA Sequence

DSPSAPVNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKE

Molecular Weight

Approximately 18-28 kDa due to the glycosylation

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

Irisin Protein, Human/Mouse/Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Irisin Protein, Human/Mouse/Rat (HEK293, His)
Cat. No.:
HY-P70664
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