Kallikrein-1 Protein, Human (240a.a, HEK293, His)

Kallikrein-1 protein cleaves bonds in kininogen to release Lys-bradykinin. It also cleaves Neisseria meningitidis NHBA in saliva during microbial infection. Kallikrein-1 Protein, Human (240a.a, HEK293, His) is the recombinant human-derived Kallikrein-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Kallikrein-1 Protein, Human (240a.a, HEK293, His) is 240 a.a., with molecular weight of ~50 kDa.

Kallikrein-1 protein functions by cleaving Met-Lys and Arg-Ser bonds in kininogen, leading to the release of Lys-bradykinin. In the context of microbial infection, it plays a role in cleaving Neisseria meningitidis NHBA in saliva, with Neisseria being an obligate commensal of the nasopharyngeal mucosa.

Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC) and the specific activity is >1,500 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Human

HEK293

C-His

P06870 (S23-S262)

3816  [NCBI]

Approximately 50 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.