KEL/Kelch-like protein 41 Protein, Human (HEK293, C-His)

KEL/Kelch-like protein 41 acts as a zinc endopeptidase with a distinct endothelin-3-converting enzyme function. It selectively cleaves endothelin-1 (EDN1), endothelin-2 (EDN2), and endothelin-3 (EDN3), displaying a preference for EDN3. KEL's endopeptidase activity plays a pivotal role in processing endothelin peptides, emphasizing its involvement in the modulation of endothelin signaling pathways. KEL/Kelch-like protein 41 Protein, Human (HEK293, C-His) is the recombinant human-derived KEL/Kelch-like protein 41 protein, expressed by HEK293 , with C-6*His labeled tag.

KEL/Kelch-like protein 41 functions as a zinc endopeptidase with specific endothelin-3-converting enzyme activity. It demonstrates the ability to cleave endothelin-1 (EDN1), endothelin-2 (EDN2), and endothelin-3 (EDN3), displaying a notable preference for EDN3 over the other substrates. Through its endopeptidase activity, KEL contributes to the processing of endothelin peptides, highlighting its role in the modulation of endothelin signaling pathways.

Human

HEK293

C-6*His

P23276 (N68-W732)

3792  [NCBI]

Extracellular Domain

NFQNCGPRPCETSVCLDLRDHYLASGNTSVAPCTDFFSFACGRAKETNNSFQELATKNKNRLRRILEVQNSWHPGSGEEKAFQFYNSCMDTLAIEAAGTGPLRQVIEELGGWRISGKWTSLNFNRTLRLLMSQYGHFPFFRAYLGPHPASPHTPVIQIDQPEFDVPLKQDQEQKIYAQIFREYLTYLNQLGTLLGGDPSKVQEHSSLSISITSRLFQFLRPLEQRRAQGKLFQMVTIDQLKEMAPAIDWLSCLQATFTPMSLSPSQSLVVHDVEYLKNMSQLVEEMLLKQRDFLQSHMILGLVVTLSPALDSQFQEARRKLSQKLRELTEQPPMPARPRWMKCVEETGTFFEPTLAALFVREAFGPSTRSAAMKLFTAIRDALITRLRNLPWMNEETQNMAQDKVAQLQVEMGASEWALKPELARQEYNDIQLGSSFLQSVLSCVRSLRARIVQSFLQPHPQHRWKVSPWDVNAYYSVSDHVVVFPAGLLQPPFFHPGYPRAVNFGAAGSIMAHELLHIFYQLLLPGGCLACDNHALQEAHLCLKRHYAAFPLPSRTSFNDSLTFLENAADVGGLAIALQAYSKRLLRHHGETVLPSLDLSPQQIFFRSYAQVMCRKPSPQDSHDTHSPPHLRVHGPLSSTPAFARYFRCARGALLNPSSRCQLW

Approximately 91.71 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.