Kirrel1/NEPH1 Protein, Mouse (HEK293, His)

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier, helping to maintain a stable podocyte structure and interdigitated foot processes connected by specialized cell-to-cell junctions called slit septa. Additionally, it acts as a signaling protein that requires the presence of TEC kinase to fully transactivate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-6*His labeled tag.

Kirrel1, also known as NEPH1, is indispensable for maintaining the proper function of the glomerular filtration barrier. It plays a crucial role in preserving a stable podocyte architecture, characterized by interdigitating foot processes connected through specialized cell-cell junctions known as the slit diaphragm. As a signaling protein, Kirrel1 requires the presence of TEC kinases for full trans-activation of the transcription factor AP-1. It engages in interactions with key proteins such as TJP1/ZO-1 and NPHS2/podocin, contributing to the intricate molecular network governing podocyte structure and function. Additionally, Kirrel1 forms homodimers through its Ig-like domains and, when tyrosine-phosphorylated, interacts with the signaling adaptor protein GRB2. Notably, its interaction with NPHS1/nephrin relies on Kirrel1 glycosylation, highlighting the complexity of its regulatory roles in podocyte biology.

Mouse

HEK293

C-6*His

Q80W68-1 (L48-L525)

170643  [NCBI]

Partial

LPGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAIPNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVYNKVGSTNVSTLVNVHFAPRIVVYPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQFAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVL

60-90 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 1 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.