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  5. LAMP1/CD107a Protein, Human (HEK293, Fc)

LAMP1/CD107a Protein, Human (HEK293, Fc)

Cat. No.: HY-P7779
Handling Instructions

LAMP1/CD107a Protein, Human (HEK293, Fc) is a recombinant human CD107a expressed in HEK 293 cells with a C-Fc tag at the C-terminus. LAMP1/CD107a Protein can be used as a functional marker for the identification of natural killer cell activity.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
10 μg $105 In-stock
Estimated Time of Arrival: December 31
50 μg $314 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

LAMP1/CD107a Protein, Human (HEK293, Fc) is a recombinant human CD107a expressed in HEK 293 cells with a C-Fc tag at the C-terminus. LAMP1/CD107a Protein can be used as a functional marker for the identification of natural killer cell activity[1][2].

Background

Lysosomal associated membrane protein 1 (LAMP1 or CD107a) is a highly glycosylated lysosomal and endosomal membrane protein of 120 kDa. LAMPs and LIMPs are tightly packed and represent more than 50% of the total membrane protein of late endosomes and lysosomes. LAMP1 has been described as a marker of CD8+ T-cell degranulation following stimulation. LAMP1 can be used as a functional marker for the identification of natural killer cell activity[1][2].

Biological Activity
Immobilized Human LGALS3 at 2μg/ml (100 μl/well) can bind recombinant Human CD107a, C-Fc (HEK293-expressed) (rHuCD107a, C-Fc). The ED50 of recombinant Human CD107a, C-Fc (HEK293-expressed) (rHuCD107a, C-Fc) is 1-5 ug/mL.
Species

Human

Source

HEK293

Accession

P11279 (A29-M382)

Gene ID
Synonyms
rHuCD107a, C-Fc; Lysosome-Associated Membrane Glycoprotein 1; LAMP-1; Lysosome-Associated Membrane Protein 1; CD107 Antigen-Like Family Member A; CD107a; LAMP1
AA Sequence

AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM-Fctag

Molecular Weight

90-130 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
LAMP1/CD107a Protein, Human (HEK293, Fc)
Cat. No.:
HY-P7779
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