LILRB4/CD85k/ILT3 Protein, Human (HEK293, hFc)

LILRB4 is a leukocyte immunoglobulin-like receptor located on chromosome 19q13.4 and has an extracellular immunoglobulin domain, a transmembrane domain and a cytoplasmic ITIM. It is expressed on immune cells, interacts with class I MHC, and inhibits the stimulation of immune responses. LILRB4/CD85k/ILT3 Protein, Human (HEK293, hFc) is the recombinant human-derived LILRB4/CD85k/ILT3 protein, expressed by HEK293 , with C-hFc labeled tag.

LILRB4, a member of the leukocyte immunoglobulin-like receptor (LIR) family, is situated in a gene cluster on chromosomal region 19q13.4. Falling within the subfamily B class of LIR receptors, it contains multiple extracellular immunoglobulin domains, a transmembrane domain, and cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). This receptor, expressed on immune cells, interacts with MHC class I molecules on antigen-presenting cells, triggering a negative signal that inhibits immune response stimulation. Additionally, LILRB4 plays a role in antigen capture and presentation, potentially influencing inflammatory responses and cytotoxicity to modulate the immune response and prevent autoreactivity. The gene exhibits broad expression across various tissues, including the appendix (RPKM 9.2), lymph nodes (RPKM 8.1), and 17 other tissues. Multiple transcript variants encoding diverse isoforms have been identified for this gene.

1.Immobilized Human ILT3, at 1 μg/mL (100 μL/well) can bind Anti-ILT3 Antibody. The ED₅₀ is 14.79 ng/mL, corresponding to a specific activity is 6.76×10⁴ U/mg.
2.Immobilized Human LILRB4 at 1 μg/mL (100 μL/well) can bind Anti-LILRB4 Antibody. The ED₅₀ for this effect is 9.525 ng/mL.
3.Immobilized Human LILRB4, hFc Tag at 0.2 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC₅₀ of ≤22.3 ng/mL determined by ELISA.

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  Immobilized Human ILT3, at 1 μg/mL (100 μL/well) can bind Anti- ILT3 Antibody, The ED₅₀ for this effect is 14.79 ng/mL, corresponding to a specific activity is 6.76×10⁴ U/mg.

Human

HEK293

C-hFc

Q8NHJ6-1/NP_001265356.2 (M21-H257)

11006  [NCBI]

Partial

MQAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEDPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRH

60-75 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/hepg2-cell.html  [Content Brief]