1. Recombinant Proteins
  2. Others
  3. LRPAP1 Protein, Human (HEK293, His)

LRPAP1 Protein, Human (HEK293, His)

Cat. No.: HY-P76478
COA Handling Instructions

The LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins and regulates ligand binding throughout the secretory pathway. It transiently interacts with the LRP1/α-2-macroglobulin receptor chain, reducing ligand binding. LRPAP1 Protein, Human (HEK293, His) is the recombinant human-derived LRPAP1 protein, expressed by HEK293 , with C-His labeled tag. The total length of LRPAP1 Protein, Human (HEK293, His) is 323 a.a., with molecular weight of ~43 KDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $95 In-stock
10 μg $160 In-stock
50 μg $420 In-stock
100 μg   Get quote  
250 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins and regulates ligand binding throughout the secretory pathway. It transiently interacts with the LRP1/α-2-macroglobulin receptor chain, reducing ligand binding. LRPAP1 Protein, Human (HEK293, His) is the recombinant human-derived LRPAP1 protein, expressed by HEK293 , with C-His labeled tag. The total length of LRPAP1 Protein, Human (HEK293, His) is 323 a.a., with molecular weight of ~43 KDa.

Background

LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins, contributing to the regulation of their ligand binding activity throughout the secretory pathway. Notably, LRPAP1 engages in transient interactions with the LRP1/alpha-2-macroglobulin receptor heavy and light chains, coinciding with a reduction in ligand binding by the receptor. Additionally, LRPAP1 forms interactions with LRP2/glycoprotein 330, LDLR, and SORL1, with diverse outcomes ranging from internalization and degradation to modulating ligand binding. The intricate network of interactions underscores the multifaceted role of LRPAP1 in orchestrating the function and trafficking of LDL receptor-related proteins.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized human LRPAP1 at 0.5 μg/mL can bind human VLDLR with a range of 3.2-400 ng/mL.

Species

Human

Source

HEK293

Tag

C-His

Accession

P30533 (Y35-L357)

Gene ID
Synonyms
Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; RAP; A2MRAP
Molecular Weight

Approximately 43 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

LRPAP1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
LRPAP1 Protein, Human (HEK293, His)
Cat. No.:
HY-P76478
Quantity:
MCE Japan Authorized Agent: