1. Recombinant Proteins
  2. Viral Proteins
  3. MERS-CoV Spike/S1 Protein, MERS-CoV (1297a.a, sf9, His)

MERS-CoV Spike/S1 Protein, MERS-CoV (1297a.a, sf9, His)

Cat. No.: HY-P73290
Handling Instructions

For research use only. We do not sell to patients.

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MERS-CoV Spike/S1 Protein, MERS-CoV (1297a.a, sf9, His) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Virus

Source

Sf9 insect cells

Tag

C-His

Accession

AFS88936.1 (M1-W1297)

Gene ID

/

Synonyms
SARS-CoV-2; Spike S1 Subunit
AA Sequence

Molecular Weight

Approximately 142.52 kDa

Purity

Greater than 85 % as determined by SDS-PAGE

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized MERS-CoV Spike/S1 Protein (1297a.a, sf9, His) at 10 μg/mL (100 μl/well) can bind DPP4 and the EC50 is 0.02-0.04 ug/mL.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 300 mM NaCl, 10% glycerol, pH7.5

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
MERS-CoV Spike/S1 Protein, MERS-CoV (1297a.a, sf9, His)
Cat. No.:
HY-P73290
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