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  2. Receptor Proteins Fluorescent-labeled Recombinant Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. FITC-Labeled Mesothelin Protein, Human (HEK293, Fc)

FITC-Labeled Mesothelin Protein, Human (HEK293, Fc)

Cat. No.: HY-P78369
COA Handling Instructions

Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. FITC-Labeled Mesothelin Protein, Human (HEK293, Fc) is the recombinant human-derived FITC-Labeled Mesothelin protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FITC-Labeled Mesothelin Protein, Human (HEK293, Fc) is 285 a.a., with molecular weight of 70-80 kDa.

For research use only. We do not sell to patients.

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Description

Mesothelin Protein, especially in its membrane-anchored forms, potentially mediates cellular adhesion, implicating its role in cell interactions. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) actively enhances in vitro megakaryocyte colony formation, indicating its participation in fostering specialized blood cell colonies' development. FITC-Labeled Mesothelin Protein, Human (HEK293, Fc) is the recombinant human-derived FITC-Labeled Mesothelin protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FITC-Labeled Mesothelin Protein, Human (HEK293, Fc) is 285 a.a., with molecular weight of 70-80 kDa.

Background

Mesothelin Protein, particularly in its membrane-anchored forms, exhibits a potential role in cellular adhesion, suggesting involvement in mediating interactions between cells. Furthermore, the associated Megakaryocyte-potentiating factor (MPF) has been identified as a potent enhancer of megakaryocyte colony formation in vitro, indicating its active participation in fostering the development of these specialized blood cell colonies.

Biological Activity

FACS Analysis of Anti-MSLN CAR Expression. 293T cells were transfected with anti-MSLN-scFv and Fc tag. Cells were stained with 5μg/ml FITC-Labeled Human MSLN (296-580) , Fc Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q13421-2 (E296-G580)

Gene ID
Molecular Construction
N-term
Mesothelin (E296-G580)
Accession # Q13421-2
hFc
C-term
Synonyms
MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
AA Sequence

EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQG

Molecular Weight

70-80 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year, protect from light. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

FITC-Labeled Mesothelin Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FITC-Labeled Mesothelin Protein, Human (HEK293, Fc)
Cat. No.:
HY-P78369
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