Myeloperoxidase/MPO Protein, Mouse (HEK293, His)

Myeloperoxidase (MPO) protein, integral to polymorphonuclear leukocytes' host defense system, catalyzes the production of hypohalous acids, notably hypochlorous acid, enhancing microbicidal activity.Beyond antimicrobial functions, MPO mediates the proteolytic cleavage of alpha-1-microglobulin, yielding t-alpha-1-microglobulin.This processed form inhibits low-density lipoprotein particle oxidation, contributing to vascular damage limitation.Myeloperoxidase/MPO Protein, Mouse (HEK293, His) is the recombinant mouse-derived Myeloperoxidase/MPO protein, expressed by HEK293 , with C-10*His labeled tag.

Myeloperoxidase (MPO) protein plays a vital role in the host defense system of polymorphonuclear leukocytes, contributing to microbicidal activity against a diverse range of organisms. Upon stimulation of polymorphonuclear leukocytes, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid under physiological conditions, along with other toxic intermediates that significantly enhance microbicidal activity in these cells. Beyond its antimicrobial functions, MPO is involved in mediating the proteolytic cleavage of alpha-1-microglobulin, generating t-alpha-1-microglobulin. This processed form exhibits potent inhibitory effects on the oxidation of low-density lipoprotein particles, thereby contributing to the limitation of vascular damage.

Measured by its ability to oxidize guaiacol in the presence of hydrogen peroxide., The specific activity is 16375.8504 pmol/min/µg, as measured under the described conditions.

Materials
Assay buffer: 20 mM MOPS, 0.1 M NaCl, 1 mM CaCl₂, pH 7.0
Test protein: Myeloperoxidase/MPO Protein, Mouse (HEK293, His) (HY-P70282)
Hydrogen Peroxide Solution, 30% (v/v) (H₂O₂)
Guaiacol（HY-N1380）

Procedure
1. Dilute guaiacol to 100 mM in assay buffer containing 0.0034% H₂O₂ to prepare the substrate mixture. Mix thoroughly by shaking or stirring at room temperature for 15 minutes. Protect from light.
2. Dilute MPO to 3.34 μg/mL in assay buffer.
3. Dilute hydrogen peroxide from 30% to 0.00667% in assay buffer.
4. In a transparent 96-well UV plate, dispense 100 μL of 3.34 μg/mL Mouse MPO, followed by 100 μL of the diluted guaiacol/H₂O₂ mixture to initiate the reaction. Mix 100 μL of assay buffer with 100 μL of the diluted guaiacol/H2O2 mixture as the substrate blank.
5. Read in kinetic mode at 470 nm for 5 minutes. Use only the data from the first 10 seconds for calculations.
6. Calculate Specific Activity:

Mouse

HEK293

C-10*His

P11247 (M16-T718)

17523  [NCBI]

Full Length of Mature Protein

MLQTSNGATPALLGEVENSVVLSCMEEAKQLVDRAYKERRESIKRSLQSGSASPTELLFYFKQPVAGTRTAVRAADYLHVALDLLKRKLQPLWPRPFNVTDVLTPAQLNLLSVSSGCAYQDVRVTCPPNDKYRTITGHCNNRRSPTLGASNRAFVRWLPAEYEDGVSMPFGWTPGVNRNGFKVPLARQVSNAIVRFPNDQLTKDQERALMFMQWGQFLDHDITLTPEPATRFSFFTGLNCETSCLQQPPCFPLKIPPNDPRIKNQKDCIPFFRSCPACTRNNITIRNQINALTSFVDASGVYGSEDPLARKLRNLTNQLGLLAINTRFQDNGRALMPFDSLHDDPCLLTNRSARIPCFLAGDMRSSEMPELTSMHTLFVREHNRLATQLKRLNPRWNGEKLYQEARKIVGAMVQIITYRDYLPLVLGPAAMKKYLPQYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLNNQYRPTGPNPRVPLSKVFFASWRVVLEGGIDPILRGLMATPAKLNRQNQIVVDEIRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPSTVGELGTVLKNLELARKLMAQYGTPNNIDIWMGGVSEPLEPNGRVGQLLACLIGTQFRKLRDGDRFWWENPGVFSKQQRQALASISLPRIICDNTGITTVSKNNIFMSNTYPRDFVSCNTLPKLNLTSWKET

Approximately 80-100 kDa due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.