N-acetylgalactosamine kinase/GALK2 Protein, Human (HEK293, His)

N-acetylgalactosamine kinase (GALK2) has dual enzymatic roles, acting on N-acetylgalactosamine (GalNAc) and functioning as a galactokinase under high galactose concentrations. GALK2 is implicated in a potential salvage pathway, facilitating the reutilization of free GalNAc from the breakdown of complex carbohydrates. N-acetylgalactosamine kinase/GALK2 Protein, Human (HEK293, His) is the recombinant human-derived N-acetylgalactosamine kinase/GALK2 protein, expressed by HEK293 , with C-6*His labeled tag.

N-acetylgalactosamine kinase (GALK2) plays a dual role in enzymatic activities, acting on N-acetylgalactosamine (GalNAc) and functioning as a galactokinase in the presence of elevated galactose concentrations. This protein is implicated in a potential salvage pathway, contributing to the reutilization of free GalNAc generated from the breakdown of complex carbohydrates.

Measured by its ability to catalyze 1mM substrate N-Acetyl-D-galactosamine and 50 μM ATP. which can be measured by absorbance at Luminescence that incubate at room temperature for 30 minutes. The specific activity is 415.58 pmol/min/μg.

Human

HEK293

C-6*His

Q01415-1 (M1-A458)

2585  [NCBI]

Full Length of Isoform-1

MATESPATRRVQVAEHPRLLKLKEMFNSKFGSIPKFYVRAPGRVNIIGEHIDYCGYSVLPMAVEQDVLIAVEPVKTYALQLANTNPLYPDFSTSANNIQIDKTKPLWHNYFLCGLKGIQEHFGLSNLTGMNCLVDGNIPPSSGLSSSSALVCCAGLVTLTVLGRNLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPLRATDVKLPSGAVFVIANSCVEMNKAATSHFNIRVMECRLAAKLLAKYKSLQWDKVLRLEEVQAKLGISLEEMLLVTEDALHPEPYNPEEICRCLGISLEELRTQILSPNTQDVLIFKLYQRAKHVYSEAARVLQFKKICEEAPENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSFLANVHKAYYQRSDGSLAPEKQSLFATKPGGGALVLLEA

Approximately 47 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, 10% Glycerol, pH 7.3.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/h4-cell.html  [Content Brief]