Neuropilin-1 Protein, Human (HEK293, Fc)

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, Fc) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Neuropilin-1 Protein, Human (HEK293, Fc) is 623 a.a., with molecular weight of 116-130 kDa.

The Neuropilin-1 protein assumes a significant role as it binds to VEGF-165, potentially inhibiting its binding to cells and inducing apoptosis by sequestering VEGF-165. This dual functionality suggests Neuropilin-1's involvement in the regulation of VEGF-mediated cellular processes. Additionally, Neuropilin-1 may interact with various members of the semaphorin family, indicating its versatility in binding to different ligands. Notably, its expression appears to exert an adverse effect on blood vessel number and integrity, suggesting a potential role in angiogenesis and vascular development. The diverse interactions and regulatory effects of Neuropilin-1 underscore its importance in orchestrating complex cellular processes and its potential implications in angiogenesis and apoptotic pathways.

Neuropilin-1 (1 nM; 15 min) activate LAP–TGF-β1 (1.4 nM) on the cell surface in MDA-MB-231 cells, and cointernalizes with it^[1].

1. Immobilized Human VEGF165 at 2 μg/mL (100 μL/well) can bind Human NRP1 Fc with a linear range of 0.2-4 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized Neuropilin-1 at 2 μg/ml (100 μl/well) can bind Biotinylated human VEGF165. The ED₅₀ for this effect is 0.01063 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Neuropilin-1 at 2 μg/ml (100 μl/well) can bind Biotinylated human VEGF165.The ED₅₀ for this effect is 0.01063 μg/mL.

Human

HEK293

C-hFc

AAH07533 (F22-K644)

8829  [NCBI]

FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK

116-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 orPBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Glinka Y, et al. Neuropilin-1 exerts co-receptor function for TGF-beta-1 on the membrane of cancer cells and enhances responses to both latent and active TGF-beta. Carcinogenesis. 2011 Apr;32(4):613-21.  [Content Brief]