Neurotrophin-3 Protein, Human (HEK293)

Neurotrophin-3 (NT-3) is crucial for promoting the survival of sensory neurons, specifically those linked to visceral and proprioceptive functions. Its role highlights NT-3's significance in maintaining and supporting the functionality of these specialized sensory cells, emphasizing its potential as a key regulator in the complex network governing essential physiological sensory processes. Neurotrophin-3 Protein, Human (HEK293) is the recombinant human-derived Neurotrophin-3 protein, expressed by HEK293 , with tag free.

Neurotrophin-3 (NT-3) emerges as a crucial factor in fostering the survival of sensory neurons, particularly those associated with visceral and proprioceptive functions. Its role in supporting the viability of these specialized sensory cells underscores the significance of NT-3 in orchestrating the intricate mechanisms that contribute to the maintenance and functionality of visceral and proprioceptive neuronal populations. The specific actions of NT-3 in promoting neuronal survival shed light on its potential as a key regulator in the complex network governing sensory processes essential for proper physiological functioning.

1. Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is < 40 ng/mL.
2. Human TrkC immobilized on CM5 Chip can bind Human NT-3 with an affinity constant of 0.0523 nM as determined in a SPR assay.

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  Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 36.23 ng/mL, corresponding to a specific activity is 2.760×10⁴ units/mg.

Human

HEK293

Tag Free

P20783 (Y139-T257)

4908

Full Length of Mature Protein

YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Approximately 14 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/mcf7-cell.html  [Content Brief]