NPR3 Protein, Human (HEK293, Fc)

NPR3 Protein, a receptor for natriuretic peptides, exhibits similar affinities for NPPA/ANP, NPPB/BNP, and NPPC/CNP. It regulates diuresis, blood pressure, and skeletal development, possibly serving as a clearance receptor for NPPA, NPPB, and NPPC. Despite lacking guanylate cyclase activity, NPR3 forms disulfide-linked homodimers and interacts with OSTN, indicating potential involvement in various physiological processes. NPR3 Protein, Human (HEK293, Fc) is the recombinant human-derived NPR3 protein, expressed by HEK293 , with C-hFc labeled tag.

The NPR3 Protein serves as a receptor for the natriuretic peptide hormones, exhibiting similar affinities for atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. It is implicated in the regulation of diuresis, blood pressure, and skeletal development and may function as a clearance receptor for NPPA, NPPB, and NPPC, thereby modulating their local concentrations and effects. Despite lacking guanylate cyclase activity, NPR3 forms homodimers that are disulfide-linked, with additional dimerization possible through the C-terminal cysteine of isoform 2. The receptor also interacts with OSTN, suggesting potential involvement in diverse physiological processes.

Human

HEK293

C-hFc

P17342-1 (T24-E481)

4883  [NCBI]

Partial

TGGGGVGGGGGGAGIGGGRQEREALPPQKIEVLVLLPQDDSYLFSLTRVRPAIEYALRSVEGNGTGRRLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAAAPVARLASHWDLPMLSAGALAAGFQHKDSEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALVYSDDKLERNCYFTLEGVHEVFQEEGLHTSIYSFDETKDLDLEDIVRNIQASERVVIMCASSDTIRSIMLVAHRHGMTSGDYAFFNIELFNSSSYGDGSWKRGDKHDFEAKQAYSSLQTVTLLRTVKPEFEKFSMEVKSSVEKQGLNMEDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVIAMTDVEAGTQEVIGDYFGKEGRFEMRPNVKYPWGPLKLRIDENRIVEHTNSSPCKSSGGLEE

Approximately 80-100 kDa, based on SDS-PAGE under reducing condition.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.