OGG1 Protein, Human

The OGG1 protein is a DNA repair enzyme specifically designed to cleave DNA at the 8-oxoG residue and plays a key role in maintaining genome integrity. It demonstrates the ability to excise damaged bases such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine from the DNA structure (FAPY). OGG1 Protein, Human (GST) is the recombinant human-derived OGG1 protein, expressed by E. coli , with tag free.

OGG1 (8-Oxoguanine DNA Glycosylase 1) is a DNA repair enzyme crucial for maintaining genomic integrity. Specializing in the repair of oxidative DNA damage, OGG1 specifically incises DNA at 8-oxoG (8-oxoguanine) residues. Additionally, it excises other oxidatively damaged bases, such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY), contributing to the removal of mutagenic lesions from the DNA. Notably, OGG1 possesses a beta-lyase activity, enabling it to nick the DNA strand 3' to the lesion, initiating the repair process. This enzymatic versatility highlights OGG1's critical role in safeguarding the genome by counteracting the detrimental effects of oxidative stress on DNA integrity.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

AAH00657.1 (M1-G345)

4968  [NCBI]

Full Length

MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG

Approximately 38.0 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/sf9-cell.html  [Content Brief]