Osteopontin/OPN Protein, Mouse (P.pastoris, His)

Osteopontin/OPN Protein, a crucial non-collagenous bone protein, binds strongly to hydroxyapatite, integral to the mineralized matrix. It likely plays a vital role in mediating cell-extracellular matrix interactions. Moreover, as a cytokine, OPN efficiently induces interferon-gamma and interleukin-12 production while inhibiting interleukin-10, pivotal in promoting type I immunity. Osteopontin/OPN Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Osteopontin/OPN protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Osteopontin/OPN Protein, Mouse (P.pastoris, His) is 274 a.a., with molecular weight (glycosylation form) of ~60 kDa.

Osteopontin (OPN) protein, a vital non-collagenous bone protein, demonstrates a strong affinity for hydroxyapatite and serves as an integral component of the mineralized matrix. It likely plays a crucial role in facilitating interactions between cells and the extracellular matrix. Additionally, OPN acts as a cytokine, effectively stimulating the production of interferon-gamma and interleukin-12, while simultaneously suppressing the production of interleukin-10. These functions are essential in the pathway that promotes type I immunity.

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10⁵ cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 ℃. The ED₅₀ for this effect is 0.5101 μg/mL, corresponding to a specific activity is 1.960×10⁴ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 105 cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.5101 μg/mL, corresponding to a specific activity is 1.960×10⁴ units/mg.

Mouse

P. pastoris

N-6*His

P10923 (L17-S290)

20750  [NCBI]

LPVKVTDSGSSEEKLYSLHPDPIATWLVPDPSQKQNLLAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVTASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVAQLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKDKLVLDPKSKEDDRYLKFRISHELESSS

Approximately 60 kDa in reducing SDS-PAGE due to glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.