PD-1 Protein, Cynomolgus (147a.a, HEK293, His)

PD-1 is a cell surface receptor on T and B cells that binds to PD-L1 and PD-L2. PD-1 is an immune checkpoint that protects autoimmunity by promoting apoptosis of antigen-specific T cells in lymph nodes (programmed cell death) and reducing apoptosis of regulatory T cells (anti-inflammatory, inhibitory T cells). The expression of PD-L1 on tumor cells inhibits anti-tumor activity through the binding of PD-1 to effector T cells. PD-1 Protein, Cynomolgus (147a.a, HEK293, His) is the recombinant cynomolgus-derived PD-1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PD-1 Protein, Cynomolgus (147a.a, HEK293, His) is 147 a.a., with molecular weight of 30-50 kDa.

PD-1 is a protein encoded by the PDCD1 gene in the human body. PD-1 is a cell surface receptor on T cells and B cells, which regulates the immune system's response to human cells by inhibiting the inflammatory activity of T cells, downregulating the immune system and promoting self-tolerance. PD-1 is an immune checkpoint that protects autoimmunity by promoting apoptosis of antigen-specific T cells in lymph nodes (programmed cell death) and reducing apoptosis of regulatory T cells (anti-inflammatory, inhibitory T cells). PD-1 can bind to two ligands, PD-L1 and PD-L2. The expression of PD-L1 on tumor cells inhibits anti-tumor activity through the binding of PD-1 to effector T cells. PD-1 knockout mice showed lupus-like glomerulonephritis and dilated cardiomyopathy in the C57BL/6 and BALB/c gene backgrounds, respectively. In the mouse thymus, the gene was induced to express in the thymus when anti-CD3 antibodies were injected and a large number of thymocytes underwent apoptosis. Mice bred in the BALB/c context lacking the gene developed dilated cardiomyopathy and died of congestive heart failure^{[1][2][3][4][5]}.

Cynomolgus

HEK293

C-6*His

B0LAJ3 (P21-Q167)

102123659  [NCBI]

PGWFLESPDRPWNAPTFSPALLLVTEGDNATFTCSFSNASESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTRLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ

30-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.