PD-1 Protein, Human (264a.a, HEK293, His)

1.Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.4-3 ng/mL.
2.Immobilized Human PD-1 Full Length, His Tag at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag with a linear range of 0.001-0.5 μg/mL.

Human

HEK293

C-His

Q15116 (L25-L288)

5133

Full length

LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL

Approximately 40-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 80%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH8.0 with detergent with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.