PHKG1 Protein, Human (Active, sf9, GST)

PHKG1 is the catalytic subunit of phosphorylase b kinase (PHK), which plays a key role in the neural and hormonal regulation of glycogenolysis (glycogen breakdown) by phosphorylating and activating glycogen phosphorylase. PHKG1 Protein, Human (Active, sf9, GST) is the recombinant human-derived PHKG1, expressed by Sf9 insect cells, with GST labeled tag.

PHKG1 (Phosphorylase Kinase Catalytic Subunit 1) is the catalytic subunit of phosphorylase b kinase (PHK), a key enzyme involved in the neural and hormonal regulation of glycogen breakdown (glycogenolysis). Its primary function is to phosphorylate and activate glycogen phosphorylase, a critical enzyme in the glycogen degradation pathway. PHKG1 has been shown to exhibit in vitro phosphorylation activity towards various substrates, including PYGM, TNNI3, MAPT/TAU, GAP43, and NRGN/RC3, suggesting a potential role in the regulation of processes beyond glycogenolysis, such as muscle function, microtubule dynamics, and neural development. It has to highlight PHKG1's central role in glycogen metabolism and hints at its broader involvement in the phosphorylation of diverse substrates, contributing to the intricate regulation of cellular functions.

Human

Sf9 insect cells

GST

Q16816-1 (T2-Y387)

5260

Partial

TRDEALPDSHSAQDFYENYEPKEILGRGVSSVVRRCIHKPTSQEYAVKVIDVTGGGSFSPEEVRELREATLKEVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLSEKETRKIMRALLEVICTLHKLNIVHRDLKPENILLDDNMNIKLTDFGFSCQLEPGERLREVCGTPSYLAPEIIECSMNEDHPGYGKEVDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLVVQPQNRYTAEEALAHPFFQQYLVEEVRHFSPRGKFKVIALTVLASVRIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAYAFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEEDY

≥ 80%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.