PLY Protein, Streptococcus pneumoniae serotype 4 (Baculovirus, His-Myc)

The PLY protein, a cholesterol-dependent toxin, induces cytolysis by forming pores in host membranes with a significant conformational change and oligomeric pore complex formation. Cholesterol is crucial for binding, insertion, and pore formation. PLY Protein, Streptococcus pneumoniae serotype 4 (Baculovirus, His-Myc) is the recombinant PLY protein, expressed by Sf9 insect cells, with N-10*His, C-Myc labeled tag. The total length of PLY Protein, Streptococcus pneumoniae serotype 4 (Baculovirus, His-Myc) is 470 a.a., with molecular weight of ~56.8 kDa.

The PLY protein operates as a cholesterol-dependent toxin, inducing cytolysis by forming pores in host membranes containing cholesterol. Following its binding to target membranes, the protein undergoes a significant conformational change, facilitating its insertion into the host membrane and the subsequent formation of an oligomeric pore complex. The presence of cholesterol is crucial for various stages of this process, including binding to host membranes, membrane insertion, and pore formation, with cholesterol binding mediated by a Thr-Leu pair in the C-terminus. Notably, PLY protein exhibits reversibility and can be inactivated by oxidation. The resulting homooligomeric pore complex, comprising 35 to 50 subunits, is formed upon insertion into the host membrane, contributing to the protein's cytolytic activity.

Others

Sf9 insect cells

N-10*His;C-Myc

P0C2J9 (A2-D471)

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ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Approximately 56.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.