PPAR gamma Protein, Human (C-His)

PPAR gamma Protein, a nuclear receptor, binds peroxisome proliferators, activating upon ligand binding to specific PPRE on DNA. It modulates target gene transcription, controlling fatty acid metabolism. Essential in adipocyte differentiation and glucose homeostasis, PPAR gamma suppresses NF-kappa-B-mediated inflammation, regulates BMAL1 in cardiovascular circadian rhythms, and modulates immune responses to M.tuberculosis by influencing MAPK p38 phosphorylation and IL-6 production. PPAR gamma Protein, Human (C-His) is the recombinant human-derived PPAR gamma protein, expressed by E. coli, with C-6*His labeled tag. The total length of PPAR gamma Protein, Human (C-His) is 266 a.a., with molecular weight of ~30 kDa.

PPAR gamma Protein, a nuclear receptor, binds to peroxisome proliferators such as hypolipidemic drugs and fatty acids. Upon ligand activation, the nuclear receptor interacts with specific PPAR response elements (PPRE) on DNA, modulating the transcription of target genes like acyl-CoA oxidase and thereby controlling the peroxisomal beta-oxidation pathway of fatty acids. It plays a pivotal role as a key regulator in adipocyte differentiation and glucose homeostasis. Additionally, PPAR gamma acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. In the context of cardiovascular circadian rhythms, it regulates the transcription of BMAL1 in blood vessels. Furthermore, in response to microbial infection, particularly treatment with M.tuberculosis or its lipoprotein LpqH, PPAR gamma modulates phosphorylation of MAPK p38 and IL-6 production, suggesting its involvement in immune responses during microbial challenges.

Data is not available.

Human

E. coli

C-6*His

P37231 (D238-D503)

5468  [NCBI]

DLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKD

Approximately 30 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of sterile 50mM Tris, 300mM NaCl, 500 mM arginine, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.