PTK7 Protein, Cynomolgus/Rhesus macaque (HEK293, His)

The PTK7 protein is an inactive tyrosine kinase that plays a key role in both non-canonical and canonical Wnt signaling pathways. Effects include cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 Protein, Cynomolgus/Rhesus macaque (HEK293, His) is the recombinant cynomolgus, Rhesus Macaque-derived PTK7 protein, expressed by HEK293 , with C-His labeled tag. The total length of PTK7 Protein, Cynomolgus/Rhesus macaque (HEK293, His) is 674 a.a., with molecular weight of 90-110 kDa.

The PTK7 protein, an inactive tyrosine kinase, plays a significant role in the Wnt signaling pathway, being a component of both the non-canonical (Wnt/planar cell polarity signaling) and canonical Wnt signaling pathways. Its involvement spans diverse cellular processes, including cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 also contributes to critical developmental events, such as embryogenesis, epithelial tissue organization, and angiogenesis. Notably, it interacts with CTNNB1, underlining its participation in Wnt pathway regulation and its impact on cellular functions with broader implications in both normal and pathological contexts.

Measured by its binding ability in a functional ELISA. Recombinant Mouse PTK7/CCK4 Fc Chimera binds Biotinylated Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 6.9 μg/mL.

Cynomolgus; Rhesus Macaque

HEK293

C-His

A0A2K5VNI5-1 (A39-T712)

102126644

AIVFIKQPSSQDALQGRRALLRCEVEAPGLVHVYWLLDGAPVQDTERRFTQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNNTVSSKERNLTLRPAGPEHSGLYSCCAHNAFGQACSSQNFTLSIADESFARVVLAPQDVIVARNEEAMFHCQFSAQPPPNLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPVILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLASIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLKKPQDSQLEEGKPGYLHCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINNVEVYDGTWYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQT

90-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.