RANKL/TNFSF11 Protein, Rat (Biotinylated, HEK293, His-Avi)

Measured by its binding ability in a functional ELISA. Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin at 1 μg/mL (100 μL/well) can bind Biotinylated Rat TNFSF11, His,Avitag with a linear range of 0.5-16 ng/mL.

Rat

HEK293

N-Avi;N-His

XP_008769150 (L69-D318)

117516

Partial

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

LYFRAQMDPNRISEDSTRCFYRILRLRENTGLQDSTLESEDTEALPDSCRRMKQAFQGAVQRELQHIVGPQRFSGVPAMMEGSWLDVARRGKPEAQPFAHLTINAANIPSGSHKVSLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPADYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISVQVSNPSLLDPDQDATYFGAFKVQDID

Approximately 35-37 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with 10% trehalose as protectant.

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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.