1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands RANKL/CD254
  5. RANKL
  6. RANK L/TNFSF11 Protein, Cynomolgus (HEK293, Fc)

RANK L/TNFSF11 Protein, Cynomolgus (HEK293, Fc)

Cat. No.: HY-P75999
Handling Instructions

For research use only. We do not sell to patients.

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  • Technical Parameters

  • Properties

  • Documentation

Species

Cynomolgus

Source

HEK 293

Tag

N-hFc

Accession

A0A7N9DBU4 (G136-D317)

Gene ID

/

Synonyms
Tumor necrosis factor ligand superfamily member 11; RANKL; CD254; ODF; OPGL; TNFSF11; TRANCE
AA Sequence

Molecular Weight

Approximately 55 kDa

Purity

Greater than 85 % as determined by reducing SDS-PAGE

Biological Activity
Immobilized cynomolgus S4-Fc3L3-TNFSF11 at 10 μg/mL (100 μL/well) can bind biotinylated human TNFRSF11B-His and the EC50 is 7.94-18.52 ng/mL. The bioactivity of RANKL was determined by measuring the ability of RANKL to induce TRAP activity in R
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
RANK L/TNFSF11 Protein, Cynomolgus (HEK293, Fc)
Cat. No.:
HY-P75999
Quantity:
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