ROBO4 Protein, Human (HEK293, Fc)

ROBO4 protein is a receptor for Slit proteins (especially SLIT2) and plays a crucial regulatory role in angiogenesis and blood vessel patterning. It mediates the inhibition of primary endothelial cell migration by Slit protein and helps regulate the angiogenic process. ROBO4 Protein, Human (HEK293, Fc) is the recombinant human-derived ROBO4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ROBO4 Protein, Human (HEK293, Fc) is 440 a.a., with molecular weight of ~87.0 kDa.

The ROBO4 Protein functions as a receptor for Slit proteins, particularly SLIT2, and appears to play a crucial role in angiogenesis and vascular patterning. It is implicated in mediating the inhibition of primary endothelial cell migration by Slit proteins, contributing to the regulation of cellular responses during angiogenic processes. Furthermore, ROBO4 is involved in maintaining the organization and function of the endothelial barrier, as evidenced by its role in endothelial barrier organization and function. The protein interacts with SLIT2 and ENAH, underscoring its specific engagement with key ligands and associated proteins in the regulation of vascular development and endothelial cell behavior.

Human

HEK293

C-hFc

Q8WZ75 (Q28-R467)

54538  [NCBI]

QDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKR

Approximately 87.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.