RSPO1/R-spondin-1 Protein, Mouse (CHO, His)

RSPO1 activates the canonical Wnt signaling pathway by binding to the LGR4-6 receptor, triggering an increase in target gene expression. It inhibits ZNRF3, regulates the Wnt signaling pathway, and negatively regulates the TGF-β pathway. RSPO1/R-spondin-1 Protein, Mouse (CHO, His) is the recombinant mouse-derived RSPO1/R-spondin-1 protein, expressed by CHO , with C-His labeled tag. The total length of RSPO1/R-spondin-1 Protein, Mouse (CHO, His) is 245 a.a., with molecular weight of ~44 kDa.

RSPO1, or R-spondin-1 protein, functions as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6, RSPO1 associates with phosphorylated LRP6 and frizzled receptors, which are activated by extracellular Wnt receptors. This association triggers the canonical Wnt signaling pathway, leading to increased expression of target genes. RSPO1 also plays a role in regulating the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by inhibiting ZNRF3, an important regulator of the Wnt signaling pathway. Additionally, RSPO1 acts as a ligand for frizzled FZD8 and LRP6, and may negatively regulate the TGF-beta pathway. It has essential roles in ovary determination and regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1. RSPO1 interacts with ZNRF3, promoting indirect interaction between ZNRF3 and LGR4 and facilitating membrane clearance of ZNRF3. It is also identified in a complex composed of RNF43, LGR5, and RSPO1. RSPO1 interacts with the extracellular domain of FZD8 and LRP6, but does not form a ternary complex with them. Additionally, RSPO1 interacts with WNT1, binds heparin, and interacts with LGR4, LGR5, and LGR6.

Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells and the ED₅₀ is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL mouse Wnt3a.

Mouse

CHO

C-His

NP_619624.2 (S21-Q265)

192199  [NCBI]

Approximately 44 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.