1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. RSPO1/R-spondin-1 Protein, Mouse (CHO, His)

RSPO1/R-spondin-1 Protein, Mouse (CHO, His)

Cat. No.: HY-P76012
COA Handling Instructions

For research use only. We do not sell to patients.

Size Price Stock Quantity
Free Sample   Apply now  
2 μg $51 In-stock
5 μg $98 In-stock
10 μg $165 In-stock
50 μg $420 Get quote
100 μg   Get quote  
250 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells and the ED50 is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL mouse Wnt3a.

Species

Mouse

Source

CHO

Tag

C-His

Accession

NP_619624.2 (S21-Q265)

Gene ID

192199  [NCBI]

Synonyms
R-spondin-1; Roof plate-specific spondin-1; RSPO1
Molecular Weight

Approximately 44 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

RSPO1/R-spondin-1 Protein, Mouse (CHO, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
RSPO1/R-spondin-1 Protein, Mouse (CHO, His)
Cat. No.:
HY-P76012
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