SARS-CoV-2 S glycoprotein (HEK293, His-mFc)

The SARS-CoV-2 S glycoprotein is critical in infection, binding to the ACE2 receptor and allowing viral particles to attach to the host cell membrane. Cleavage of S2/S2′ triggers cell membrane fusion or internalization via endocytosis. SARS-CoV-2 S glycoprotein (HEK293, His-mFc) is the recombinant Virus-derived SARS-CoV-2 S glycoprotein, expressed by HEK293 , with C-mFc, C-6*His labeled tag. The total length of SARS-CoV-2 S glycoprotein (HEK293, His-mFc) is 223 a.a., with molecular weight (glycosylation form) of ~66.0 kDa.

The SARS-CoV-2 S glycoprotein plays a crucial role in infection by attaching the virion to the host cell membrane through interaction with the primary receptor, host ACE2. Upon cleavage of S2/S2', binding to the ACE2 receptor initiates either direct fusion at the cell membrane or internalization of the virus via endocytosis, leading to fusion of the virion membrane with the host endosomal membrane. Additionally, the glycoprotein may utilize NRP1/NRP2 and integrin as alternative entry receptors, possibly explaining the virus's tropism in human olfactory epithelial cells. The stalk domain of S exhibits three hinges, providing unexpected orientational freedom to the head. Acting as a class I viral fusion protein, the glycoprotein undergoes an extensive and irreversible conformational change during virus entry, triggered by host TMPRSS2 or CSTL, leading to fusion of the viral envelope with the cellular cytoplasmic membrane and release of viral genomic RNA into the host cell cytoplasm. The glycoprotein exhibits at least three conformational states: pre-fusion native, pre-hairpin intermediate, and post-fusion hairpin, with the coiled coil regions adopting a trimer-of-hairpins structure during fusion, facilitating the apposition and subsequent fusion of viral and target cell membranes.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 0.38147-50000 μg/mL can bind SARS-CoV-2-S1-RBD at 0.2 μg/well, the EC₅₀ of SARS-CoV-2-S1-RBD protein is 26.45-45.47 ng/mL.

Virus

HEK293

C-mFc;C-6*His

P0DTC2 (R319-F541)

43740568  [NCBI]

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Approximately 66.0 kDa.The reducing (R) protein migrat es as 66 kDa in SDS-PAGE may be due to glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.