SARS-CoV-2 S Protein RBD (194a.a, HEK293, His)

SARS-CoV-2 S Protein RBD (HEK293, His) produced in HEK293 cells is a recombinant SARS-CoV S protein receptor-binding domain (RBD) with His tag.

The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. A fragment that is located in the S1 subunit and spans amino acids 318–510 is the minimal receptor-binding domain (RBD).

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/mL (100 μL/well) on the plate can bind SARS-CoV-2 S RBD. The ED₅₀ for this effect is <0.25 μg/mL.

Virus

HEK293

C-6*His

QHD43416.1 (N331-V524)

43740568  [NCBI]

Partial

NITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATV

28-35 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Lanying Du, et al. The spike protein of SARS-CoV — a target for vaccine and therapeutic development. Nat Rev Microbiol. 2009; 7(3): 226–236.  [Content Brief]