SARS-CoV-2 S Protein RBD (194a.a, HEK293, His)
Based on 1 Customer Validation
SARS-CoV-2 S Protein RBD (HEK293, His) produced in HEK293 cells is a recombinant SARS-CoV S protein receptor-binding domain (RBD) with His tag.
- Species: Virus
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
SARS-CoV-2 S Protein RBD (HEK293, His) produced in HEK293 cells is a recombinant SARS-CoV S protein receptor-binding domain (RBD) with His tag.
Background
The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. A fragment that is located in the S1 subunit and spans amino acids 318–510 is the minimal receptor-binding domain (RBD).
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 (HY-P7443) at 2 μg/mL (100 μL/well) on the plate can bind SARS-CoV-2 S RBD. The ED50 for this effect is <0.25 μg/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Virus
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Source HEK293
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Tag C-6*His
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Accession
QHD43416.1 (N331-V524)
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Molecular Construction
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N-term
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Spike RBD (N331-V524)
Accession # QHD43416.1 -
6*His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein; S protein RBD; 2019-nCoV S protein RBD
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AA Sequence
NITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATV
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Predicted Molecular Mass
22.6 kDa
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Molecular Weight
Approximately 28-35 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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