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SARS-CoV-2 S Protein RBD (HEK293)

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Based on 1 Customer Validation

The SARS-Cov-2 S glycoprotein is a SARS-Cov-2 S glycoprotein. The amino acid sequence 1261-1267a.a of the SARS-Cov-2 S glycoprotein is transmembrane. The SARS-Cov-2 S glycoprotein is a highly glycosylated trimer, each of which consists of 1260 amino acids (residues 14-1273), divided into four structural domains: the n-terminal domain (NTD), the c-terminal domain (CTD, also known as the receptor-binding domain, RBD), and two subdomains (SD1 and SD2). SARS-CoV-2 S Protein RBD (HEK293) is the recombinant Virus-derived SARS-CoV-2 S protein RBD, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Virus
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SARS-CoV-2 S Protein RBD (HEK293)
  • Experimental Validation Results for SARS-CoV-2 S Protein RBD (HEK293)
  • SARS-CoV-2 S Protein RBD (HEK293) Product Image
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[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The SARS-Cov-2 S glycoprotein is a SARS-Cov-2 S glycoprotein. The amino acid sequence 1261-1267a.a of the SARS-Cov-2 S glycoprotein is transmembrane. The SARS-Cov-2 S glycoprotein is a highly glycosylated trimer, each of which consists of 1260 amino acids (residues 14-1273), divided into four structural domains: the n-terminal domain (NTD), the c-terminal domain (CTD, also known as the receptor-binding domain, RBD), and two subdomains (SD1 and SD2). SARS-CoV-2 S Protein RBD (HEK293) is the recombinant Virus-derived SARS-CoV-2 S protein RBD, expressed by HEK293 , with tag free.

Background

SARS-Cov-2 is a enveloped positive-sense single-stranded RNA virus that causes COVID-19.
SARS-CoV-2 possesses four structural proteins, namely the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M), and nucleocapsid protein (N).
The SARS-Cov-2 S glycoprotein is located on the exterior of the viral particle, giving the coronavirus its crown-like appearance.
The SARS-Cov-2 S glycoprotein can mediate the attachment and entry of viral particles into host cells and is an important target for vaccine development, antibody therapy, and antigen-based diagnostic esting[1][2][3][4][5].

Verified Bioactivity

1.Immobilized SARS-CoV-2 S Protein RBD at 2 μg/mL (100 μL/well) can bind ACE2 Protein, Human (mFc) and the EC50 is 20-80 ng/mL.
2.Immobilized Human ACE2 at 2 μg/mL (100 μL/well) can bind Biotinylated Virus SARS-CoV-2 S protein. The ED50 for this effect is 10-60 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Human ACE2 at 2 μg/mL (100 μL/well) can bind biotinylated SARS-CoV-2 S protein. The ED50 for this effect is 10.95 ng/mL, corresponding to a specific activity is 9.13×104 Unit/mg.

Technical Parameters

  • Species Virus
  • Source HEK293
  • Tag Tag Free
  • Accession

    YP_009724390.1 (R319-F541)

  • Gene ID
    43740568  [NCBI]
  • Molecular Construction
    • N-term
    • Spike RBD (R319-F541)
      Accession # YP_009724390.1
    • C-term
  • Protein Length

    Full Length of Receptor-binding domain (RBD) Region

  • Synonyms

    2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein; S protein RBD; 2019-nCoV S protein RBD

  • AA Sequence

    RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

  • Molecular Weight

    Approximately 34 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Human ACE2 at 2 μg/mL (100 μL/well) can bind biotinylated SARS-CoV-2 S protein. The ED50 for this effect is 10.95 ng/mL, corresponding to a specific activity is 9.13×104 Unit/mg.

  • SARS-CoV-2 S Protein RBD (HEK293) Product Image
    1/16
  • Experimental Validation Results for SARS-CoV-2 S Protein RBD (HEK293)
  • Experimental Validation Results for SARS-CoV-2 S Protein RBD (HEK293)
  • SARS-CoV-2 S Protein RBD (HEK293) Product Image