SARS-CoV-2 S Protein RBD (HEK293)

1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 S Protein RBD at 2 μg/mL (100 μL/well) can bind ACE2 Protein, Human (mFc) and the EC₅₀ is 20-80 ng/mL.
2. Immobilized Human ACE2 at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein. The ED₅₀ for this effect is 10.95 ng/mL, corresponding to a specific activity is 9.13×10⁴ Unit/mg.

• 
  Immobilized Human ACE2 at 2μg/mL (100 μL/well) can bind SARS-CoV-2 S protein. The ED₅₀ for this effect is 10.95ng/mL, corresponding to a specific activity is 9.13×10⁴ Unit/mg.

Virus

HEK293

Tag Free

YP_009724390.1 (R319-F541)

43740568  [NCBI]

RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

approximately 34 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.