1. Recombinant Proteins
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV Nucleoprotein/NP Protein (sf9, His)

SARS-CoV Nucleoprotein/NP Protein (sf9, His)

Cat. No.: HY-P74582
Handling Instructions

SARS-CoV Nucleoprotein (NP, N) is a homodimer with nonspecific binding activity toward nucleic acids. NP packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly, enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication through its interactions with the viral genome and membrane protein M. SARS-CoV Nucleoprotein/NP Protein (sf9, His) is the recombinant Virus-derived SARS-CoV Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SARS-CoV Nucleoprotein/NP Protein (sf9, His) is 422 a.a., with molecular weight of ~47.1 kDa.

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Description

SARS-CoV Nucleoprotein (NP, N) is a homodimer with nonspecific binding activity toward nucleic acids. NP packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly, enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication through its interactions with the viral genome and membrane protein M. SARS-CoV Nucleoprotein/NP Protein (sf9, His) is the recombinant Virus-derived SARS-CoV Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SARS-CoV Nucleoprotein/NP Protein (sf9, His) is 422 a.a., with molecular weight of ~47.1 kDa.

Background

SARS-CoV Nucleoprotein (NP, N) is a homodimer composed of 422 amino acids in each chain. NP can be divided into two structural domains interspersed with disordered (unstructured) regions: the N-terminal domain (NTD; also called RBD) serves as a putative RNA-binding domain, while the C-terminal domain (CTD; also called DD) is a dimerization domain, both the NTD and the CTD bind to nucleic acids through electropositive regions on their surfaces.
NP is one of the most abundant coronavirus proteins with nonspecific binding activity toward nucleic acids, including ssRNA, single-stranded DNA, and double-stranded DNA, NP can also act as an RNA chaperone. NP packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. NP plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication[1].

Species

Virus

Source

Sf9 insect cells

Tag

C-His

Accession

NP_828858 (M1-A422)

Gene ID

1489678  [NCBI]

Molecular Construction
N-term
Nucleoprotein (M1-A422)
Accession # NP_828858
His
C-term
Synonyms
Nucleoprotein; Ebola NP; eNP; Protein N; NP
Molecular Weight

Approximately 47.1 kDa

Purity

Greater than 80% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

SARS-CoV Nucleoprotein/NP Protein (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
SARS-CoV Nucleoprotein/NP Protein (sf9, His)
Cat. No.:
HY-P74582
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