Semaphorin-4C/SEMA4C Protein, Mouse (HEK293, Fc)

Semaphorin-4C/SEMA4C is the cell surface receptor of PLXNB2 and is critical for intercellular signaling.Semaphorin-4C/SEMA4C Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Semaphorin-4C/SEMA4C protein, expressed by HEK293 , with C-hFc labeled tag.

Semaphorin-4C (SEMA4C) acts as a cell surface receptor for PLXNB2, serving a crucial role in cell-cell signaling. The binding of SEMA4C to PLXNB2 initiates downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. This protein is essential for normal brain development, axon guidance, and cell migration. Additionally, SEMA4C is a likely signaling receptor that may contribute to myogenic differentiation through the activation of the stress-activated MAPK cascade. Notably, it engages in interactions with GIPC via its PDZ-binding motif and PDZ domain, as well as with NCDN and DLG4. The multifaceted functions of SEMA4C underscore its significance in coordinating various cellular processes.

Mouse

HEK293

C-hFc

Q64151 (A21-G664）

20353  [NCBI]

Extracellular Domain

AEMWWNLVPRKTVSSGELVTVVRRFSQTGIQDFLTLTLTEHSGLLYVGAREALFAFSVEALELQGAISWEAPAEKKIECTQKGKSNQTECFNFIRFLQPYNSSHLYVCGTYAFQPKCTYINMLTFTLDRAEFEDGKGKCPYDPAKGHTGLLVDGELYSATLNNFLGTEPVILRYMGTHHSIKTEYLAFWLNEPHFVGSAFVPESVGSFTGDDDKIYFFFSERAVEYDCYSEQVVARVARVCKGDMGGARTLQKKWTTFLKARLVCSAPDWKVYFNQLKAVHTLRGASWHNTTFFGVFQARWGDMDLSAVCEYQLEQIQQVFEGPYKEYSEQAQKWARYTDPVPSPRPGSCINNWHRDNGYTSSLELPDNTLNFIKKHPLMEDQVKPRLGRPLLVKKNTNFTHVVADRVPGLDGATYTVLFIGTGDGWLLKAVSLGPWIHMVEELQVFDQEPVESLVLSQSKKVLFAGSRSQLVQLSLADCTKYRFCVDCVLARDPYCAWNVNTSRCVATTSGRSGSFLVQHVANLDTSKMCNQYGIKKVRSIPKNITVVSGTDLVLPCHLSSNLAHAHWTFGSQDLPAEQPGSFLYDTGLQALVVMAAQSRHSGPYRCYSEEQGTRLAAESYLVAVVAGSSVTLEARAPLENLG

Approximately 115-125 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.