Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, Fc)

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling. It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner. Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, Fc) is 688 a.a., with molecular weight of 100-130 kDa.

Semaphorin-4D (SEMA4D) operates as a cell surface receptor for PLXNB1 and PLXNB2, assuming a crucial role in cell-cell signaling. This versatile protein is implicated in diverse cellular processes, including the regulation of GABAergic synapse development, where it fosters the development of inhibitory synapses in a PLXNB1-dependent manner. In hippocampal neurons, SEMA4D influences the complexity and arborization of developing neurites by activating PLXNB1, thereby mediating RHOA activation. Additionally, SEMA4D is involved in promoting the migration of cerebellar granule cells and induces B-cell aggregation, enhancing their viability in vitro. Furthermore, SEMA4D stimulates endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. Operating as a homodimer, SEMA4D interacts with both PLXNB1 and PLXNB2, underscoring its intricate involvement in various cellular and developmental processes.

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ this effect is 0.4301 μg/mL, corresponding to a specific activity is 2.33×10³ units/mg.

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  Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is 0.4301 μg/mL, corresponding to a specific activity is 2.33×10³ units/mg.

Mouse

HEK293

C-hFc

O09126 (F24-M711)

20354  [NCBI]

FAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKM

100-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4 or PBS.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.