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  4. Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc)

Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc)

Cat. No.: HY-P74560
Handling Instructions

Semaphorin-5A/SEMA5A Protein acts as a bifunctional axonal guidance cue, with attractive effects via heparan sulfate proteoglycans and inhibitory effects through chondroitin sulfate proteoglycans. As a ligand for PLXNB3 receptor, SEMA5A disrupts glioma cell migration and invasion, inducing cellular collapse. It may also promote angiogenesis by affecting endothelial cell proliferation and migration, while inhibiting apoptosis. SEMA5A's binding to PLXNB3 reveals its diverse roles in axon guidance, cell migration, and angiogenesis in both normal and pathological conditions. Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc) is the recombinant human-derived Semaphorin-5A/SEMA5A protein, expressed by HEK293, with C-hFc labeled tag. The total length of Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc) is 765 a.a., with molecular weight of 125-135 kDa.

For research use only. We do not sell to patients.

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Description

Semaphorin-5A/SEMA5A Protein acts as a bifunctional axonal guidance cue, with attractive effects via heparan sulfate proteoglycans and inhibitory effects through chondroitin sulfate proteoglycans. As a ligand for PLXNB3 receptor, SEMA5A disrupts glioma cell migration and invasion, inducing cellular collapse. It may also promote angiogenesis by affecting endothelial cell proliferation and migration, while inhibiting apoptosis. SEMA5A's binding to PLXNB3 reveals its diverse roles in axon guidance, cell migration, and angiogenesis in both normal and pathological conditions. Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc) is the recombinant human-derived Semaphorin-5A/SEMA5A protein, expressed by HEK293, with C-hFc labeled tag. The total length of Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc) is 765 a.a., with molecular weight of 125-135 kDa.
Background

Semaphorin-5A/SEMA5A Protein functions as a bifunctional axonal guidance cue regulated by sulfated proteoglycans, exhibiting attractive effects through interactions with heparan sulfate proteoglycans (HSPGs), and inhibitory effects dependent on interactions with chondroitin sulfate proteoglycans (CSPGs). As a ligand for receptor PLXNB3, SEMA5A plays a crucial role in glioma cells, where its stimulation of PLXNB3 leads to the disassembly of F-actin stress fibers, disruption of focal adhesions, cellular collapse, and the inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. Furthermore, SEMA5A may promote angiogenesis by enhancing endothelial cell proliferation and migration while inhibiting apoptosis. The binding of SEMA5A to PLXNB3 underscores its involvement in diverse cellular processes, providing valuable insights into its potential roles in both normal development and pathological conditions, particularly in the context of axon guidance, cell migration, and angiogenesis.
Species

Human
Source

HEK293
Tag

C-hFc
Accession

Q13591 (M1-T765)
Gene ID
Molecular Construction
N-term
SEMA5A (M1-T765)
Accession # Q13591
hFc
C-term
Synonyms
Semaphorin-5A; Semaphorin-F; Sema F; SEMA5A; SEMAF
Molecular Weight

125-135 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Semaphorin-5A/SEMA5A Protein, Human (HEK293, Fc) Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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