STX7 Protein, Human (HEK293, His)

The STX7 protein plays a key role in protein trafficking and membrane fusion events. It guides proteins from the plasma membrane to early endosomes, facilitates homotypic fusion of endocytic organelles, and mediates transport from early endosomes to late endosomes and lysosomes. STX7 Protein, Human (HEK293, His) is the recombinant human-derived STX7 protein, expressed by HEK293 , with N-6*His labeled tag.

STX7 Protein emerges as a pivotal player in cellular processes governing protein trafficking and membrane fusion events. It is implicated in the trafficking of proteins from the plasma membrane to the early endosome (EE) and contributes to the homotypic fusion of endocytic organelles. Additionally, STX7 mediates endocytic trafficking from early endosomes to late endosomes and lysosomes, emphasizing its role in the dynamic regulation of endosomal compartments. By forming a SNARE complex with VTI1B, STX8, and VAMP8, STX7 plays a crucial role in the homotypic fusion of late endosomes. It is also a component of the SNARE complex, along with STX8, VAMP7, and VTI1B, essential for the heterotypic fusion of late endosomes with lysosomes. Interactions with VPS11, VPS16, VPS18, VPS33A, and TPC1 underscore the intricate network of associations, revealing the multifaceted involvement of STX7 in coordinating membrane fusion events and protein trafficking within the endocytic pathway. Investigating the detailed mechanisms underlying STX7's functions could provide valuable insights into its regulatory role in cellular membrane dynamics.

Human

HEK293

N-6*His

O15400 (S2-L238)

8417  [NCBI]

Partial

SYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTL

Approximately 31-36 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.