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  3. Testican 1/SPOCK1 Protein, Human (sf9, His)

Testican 1/SPOCK1 Protein, Human (sf9, His)

Cat. No.: HY-P76654
COA Handling Instructions

The Testican 1/SPOCK1 protein may play a role in cell-cell and cell-matrix interactions, suggesting involvement in fundamental cellular processes related to intercellular communication and adhesion. Furthermore, it may contribute to various neuronal mechanisms in the central nervous system, suggesting a potential role in complex processes controlling neuronal function. Testican 1/SPOCK1 Protein, Human (sf9, His) is the recombinant human-derived Testican 1/SPOCK1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Testican 1/SPOCK1 Protein, Human (sf9, His) is 439 a.a., with molecular weight of ~53 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The Testican 1/SPOCK1 protein may play a role in cell-cell and cell-matrix interactions, suggesting involvement in fundamental cellular processes related to intercellular communication and adhesion. Furthermore, it may contribute to various neuronal mechanisms in the central nervous system, suggesting a potential role in complex processes controlling neuronal function. Testican 1/SPOCK1 Protein, Human (sf9, His) is the recombinant human-derived Testican 1/SPOCK1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Testican 1/SPOCK1 Protein, Human (sf9, His) is 439 a.a., with molecular weight of ~53 kDa.

Background

The Testican 1/SPOCK1 protein is implicated in potentially playing a role in cell-cell and cell-matrix interactions, suggesting its involvement in fundamental cellular processes related to intercellular communication and adhesion. Additionally, Testican 1/SPOCK1 may contribute to various neuronal mechanisms in the central nervous system, indicating a potential role in the intricate processes that govern neuronal function. The specific mechanisms and contexts in which Testican 1/SPOCK1 operates in regulating cell interactions and neuronal processes remain areas of interest, underscoring its potential significance in cellular dynamics and neural function.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

Q08629 (M1-W439)

Gene ID
Molecular Construction
N-term
SPOCK1 (M1-W439)
Accession # Q08629
His
C-term
Synonyms
Testican-1; Protein SPOCK; TIC1; TICN1; SPOCK
Molecular Weight

Approximately 53 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Testican 1/SPOCK1 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Testican 1/SPOCK1 Protein, Human (sf9, His)
Cat. No.:
HY-P76654
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