Latent TGF beta 1/TGFB1 Protein, Mouse (HEK293, His)

Transforming growth factor beta-1 proprotein is the precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains. TGF beta 1/TGFB1 Protein, Mouse (HEK293, His) is a recombinant protein (L30-S390) produced by HEK293 cells with His tag^[1][2].

Transforming growth factor beta-1 proprotein is the precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. The pro-protein is enzymatically cleaved to generate an amino-terminal Latency Associated Peptide (LAP), but this product remains non-covalently associated with TGFβ-1 keeping it in a biologically inactive state.

1. Immobilized Mouse Latent TGF beta 1, His Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, hFc Tag with the EC₅₀ of 0.39-0.43 μg/mL determined by ELISA.
2. Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED₅₀ for this effect is ≤0.2066 ng/mL, corresponding to a specific activity is ≥4.84×10⁶ units/mg.

• 
  Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. The ED₅₀ for this effect is 0.1173 ng/mL, corresponding to a specific activity is 8.525×10⁶ units/mg.

Mouse

HEK293

N-His

P04202 (L30-S390)

21803  [NCBI]

Full Length of Mature Protein

LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYEKTKDISHSIYMFFNTSDIREAVPEPPLLSRAELRLQRLKSSVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNKLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Approximately 40-50 kDa & 14-15 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. K. Miyazono, et al. A role of the latent TGF-beta 1-binding protein in the assembly and secretion of TGF-beta 1. The EMBO Journal (1991)10:1091-1101.

  [2]. M Selvakumaran, et al. The novel primary response gene MyD118 and the proto-oncogenes myb, myc, and bcl-2 modulate transforming growth factor beta 1-induced apoptosis of myeloid leukemia cells. Mol Cell Biol. 1994 Apr;14(4):2352-60.  [Content Brief]

  [3]. Chambaz E.M., et al. Transforming Growth Factor-βs: A Multifunctional Cytokine Family. Implication in the Regulation of Adrenocortical Cell Endocrine Functions. 1991.

  [4]. Dulce Maroni, et al. TGFB1 disrupts the angiogenic potential of microvascular endothelial cells of the corpus luteum. Cell Sci. 2011 Jul 15;124(Pt 14):2501-10.  [Content Brief]

  [5]. Justin Rustenhoven, et al. TGF-beta1 regulates human brain pericyte inflammatory processes involved in neurovasculature function. Justin Rustenhoven. 2016 Feb 11;13:37.  [Content Brief]

  [6]. Kai Zhang, et al. Essential role of microglial transforming growth factor-β1 in antidepressant actions of (R)-ketamine and the novel antidepressant TGF-β1. Transl Psychiatry. 2020 Jan 27;10(1):32.  [Content Brief]