TrkA Protein, Human (HEK293, His)

Pentraxin 3/TSG-14 protein is a multifunctional protein that plays a role in immune response and inflammation. It is involved in the recognition and clearance of pathogens, as well as regulation of inflammatory processes. Dysregulation of Pentraxin 3/TSG-14 protein has been associated with various diseases, including cardiovascular disorders and infections. Targeting Pentraxin 3/TSG-14 protein may hold therapeutic potential in these conditions. TrkA Protein, Human (HEK293, His) is the recombinant human-derived TrkA protein, expressed by HEK293 , with N-His labeled tag. The total length of TrkA Protein, Human (HEK293, His) is 220 a.a., with molecular weight of 35-60 kDa.

The TrkA Protein, a member of the neurotrophic tyrosine kinase receptor (NTKR) family, encodes a membrane-bound receptor that, upon neurotrophin binding, initiates phosphorylation of itself and members of the MAPK pathway. This kinase's presence facilitates cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been linked to congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability, and cancer. Several alternate transcriptional splice variants of this gene have been identified, but only three have been characterized to date. The gene demonstrates biased expression, with higher levels detected in the adrenal (RPKM 3.7), testis (RPKM 1.0), and 10 other tissues, suggesting its potential significance in various physiological contexts across multiple organs.

Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.06643 μg/mL, corresponding to a specific activity is 1.51×10^4 units/mg.

Human

HEK293

N-His

NP_002520.2 (P194-E413)

4914  [NCBI]

PTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTCWAENDVGRAEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTEFLEPAANETVRHGCLRLNQPTHVNNGNYTLLAANPFGQASASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSGDPVEKKDE

35-60 kDa

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  Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.06643 μg/mL, corresponding to a specific activity is 1.51×10₄ units/mg.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.