Tyrosine Hydroxylase Protein, Human (sf9, His)
Based on 1 Customer Validation
Tyrosine hydroxylase is a key enzyme in the biosynthesis of catecholamines, catalyzing the conversion of L-tyrosine into L-dopa, which is the rate-limiting step in the synthesis of dopamine, norepinephrine, and epinephrine. Tyrosine is hydroxylated using tetrahydrobiopterin and oxygen. Tyrosine Hydroxylase Protein, Human (sf9, His) is the recombinant human-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Tyrosine hydroxylase is a key enzyme in the biosynthesis of catecholamines, catalyzing the conversion of L-tyrosine into L-dopa, which is the rate-limiting step in the synthesis of dopamine, norepinephrine, and epinephrine. Tyrosine is hydroxylated using tetrahydrobiopterin and oxygen. Tyrosine Hydroxylase Protein, Human (sf9, His) is the recombinant human-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
Tyrosine Hydroxylase, a pivotal enzyme in the biosynthesis of catecholamines, facilitates the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), representing the rate-limiting step in the synthesis of neurotransmitters such as dopamine, noradrenaline, and adrenaline. Utilizing tetrahydrobiopterin and molecular oxygen, the enzyme orchestrates the hydroxylation of tyrosine to form L-Dopa, a critical precursor in catecholamine biosynthesis. While tyrosine is its primary substrate, the enzyme exhibits a broader substrate range, catalyzing the hydroxylation of phenylalanine and tryptophan with lower specificity. Moreover, Tyrosine Hydroxylase contributes to the positive regulation of the regression of retinal hyaloid vessels during postnatal development. Despite its central role in catecholamine synthesis, it is worth noting that the discussed instance of Tyrosine Hydroxylase lacks catalytic activity.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
P07101-3 (P2-G497)
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Molecular Construction
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N-term
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His
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TyrH (P2-G497)
Accession # P07101-3 -
C-term
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Protein Length
Partial
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Synonyms
TH; TYH; Tyrosine Hydroxylase; Dystonia 14; Tyrosine 3-Monooxygenase; TH Protein; DYT5b; DYT14; Tyrosine 3-Hydroxylase
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AA Sequence
PTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAVLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEEPEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFSVKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG
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Predicted Molecular Mass
57.6 kDa
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Molecular Weight
Approximately 55-66 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol, 5% trehalose, 5% mannitol and 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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