USAG-1 Protein, Human (HEK293, Fc)

USAG-1 protein is involved in initiating endometrial receptivity, contributing to implantation preparation and decidual cell response sensitization. It regulates signaling pathways by enhancing Wnt signaling while inhibiting TGF-β signaling. USAG-1 Protein, Human (HEK293, Fc) is the recombinant human-derived USAG-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of USAG-1 Protein, Human (HEK293, Fc) is 183 a.a., with molecular weight of 55-70 kDa.

USAG-1 protein appears to be implicated in the initiation of endometrial receptivity, potentially contributing to the preparation for implantation and sensitization for the decidual cell reaction. Moreover, it plays a role in modulating signaling pathways by enhancing Wnt signaling and concurrently inhibiting TGF-beta signaling. Notably, USAG-1 acts as a direct antagonist to BMP2, BMP4, BMP6, and BMP7 in a dose-dependent manner, suggesting a regulatory function in the BMP signaling pathway. It interacts with high affinity with BMP2, BMP4, BMP6, and BMP7, indicating a direct involvement in the modulation of these signaling molecules.

Measured by its binding ability in a functional ELISA. When Recombinant Human USAG1 is coated at 2 μg/mL (100 μL/well), the concentration of rhLRP-6 that produces 50% of the optimal binding response is found to be approximately 0.0323 μg/mL.

Human

HEK293

C-hFc

Q6X4U4-1 (F24-S206)

/

FKNDATEILYSHVVKPVPAHPSSNSTLNQARNGGRHFSNTGLDRNTRVQVGCRELRSTKYISDGQCTSISPLKELVCAGECLPLPVLPNWIGGGYGTKYWSRRSSQEWRCVNDKTRTQRIQLQCQDGSTRTYKITVVTACKCKRYTRQHNESSHNFESMSPAKPVQHHRERKRASKSSKHSMS

Approximately 55-70 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.