USP2 Catalytic Domain Protein, Human

USP2 catalytic domain proteins act as hydrolases and target polyubiquitinated proteins such as MDM2, MDM4, and CCND1. Both isoform 1 and isoform 4 exhibit ubiquitin-specific peptidase and isopeptidase activities. USP2 Protein, Human is the recombinant human-derived USP2 protein, expressed by E. coli , with tag free.

The USP2 catalytic domain protein acts as a hydrolase, targeting polyubiquitinated proteins such as MDM2, MDM4, and CCND1. Isoform 1 and isoform 4 exhibit both ubiquitin-specific peptidase and isopeptidase activities. Notably, it deubiquitinates MDM2, indirectly promoting p53/TP53 degradation and limiting p53 activity without reversing MDM2-mediated ubiquitination of p53. Additionally, USP2 prevents MDM2-mediated degradation of MDM4, contributing to the intricate regulation of cellular processes. In the context of cell-cycle progression, USP2 plays a role in G1/S transition in both normal and cancer cells. Furthermore, it emerges as a regulator of the circadian clock, influencing its intrinsic rhythm and responsiveness to external cues. By associating with clock proteins, USP2 deubiquitinates the core clock component PER1, affecting its nucleocytoplasmic shuttling and nuclear retention. This multifaceted protein also participates in the regulation of myogenic differentiation of embryonic muscle cells and serves as a circadian clock output effector, influencing Ca(2+) absorption in the small intestine by likely modulating NHERF4 levels and, consequently, the activity of the Ca(2+) channel TRPV6.

The fundamental role of USP2 is specific removal of ubiquitin from substrates. USP2 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

E. coli

Tag Free

O75604 (N259-M605)

9099

Partial

NSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 500 mM NaCl, 5% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.