ZAP70 Protein, Human (Active, sf9, His, GST)

ZAP70 Protein, Human (sf9, His, GST) is the recombinant human-derived ZAP70, expressed by Sf9 insect cells , with His, GST labeled tag. ,

ZAP70 is a tyrosine kinase essential for regulating adaptive immune responses. It controls motility, adhesion, and cytokine expression in mature T-cells, thymocyte development, and contributes to primary B-lymphocyte activation. Upon TCR stimulation by APCs, phosphorylation cascades recruit ZAP70 to doubly phosphorylated CD247/CD3Z via ITAM motifs, localizing it to activated TCR and releasing its autoinhibited conformation—further stabilized by LCK-mediated phosphorylation. ZAP70 then phosphorylates adapter proteins LAT and LCP2, initiating signaling cascades that drive lymphokine production, T-cell proliferation, and differentiation. It also modulates T-cell cytoskeleton dynamics for effector delivery to APCs and regulates TCR internalization/degradation via interactions with CBL, SLA, and SLA2, thereby controlling TCR surface expression for activation modulation. During thymocyte development, ZAP70 promotes survival and cell-cycle progression in CD4/CD8 double-negative stages. By similar mechanisms, it may support primary B-cell formation/activation through BCR signaling.

The activity of ZAP70 is verified by the ADP-Glo method: Incubate ZAP70 protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.

Human

Sf9 insect cells

His;GST

P43403-1 (P2-A619)

7535

Full Length of Isoform 1

PDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.