RNase H2 

RNase H2 is the predominant source of RNase H activity in mammalian and human cells. RNase H2 protects genome integrity. RNase H2 has been associated with ribonucleotide removal from genomic DNA in yeast and mouse, where it is required for embryonic development^[1][2].

Product Information
This product is recombinantly expressed in E. coli. In addition to degrading the RNA chain in the R-loop and DNA-RNA complex chain, it can also specifically cut single ribonucleotides in the DNA double-stranded chain to repair the DNA double-stranded chain.
Storage buffer: 20 mM Tris-HCl, 100 mM KCI, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 8.0

Instructions
1. Add 100 pmol double-stranded DNA substrate, 0.2-0.5 μL RNase H2, 5 μL reaction buffer (200 mM Tris-HCl, 100 mM (NH₄) ₂SO₄, 100 mM KCl, 20 mM MgSO₄, 1% Triton X-100, pH 8.8) according to the reaction system, and add Nuclease-free Water to make up to 50 μL;
2. After mixing, incubate the sample at 37°C for 30 min.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Liquid

Colorless to light yellow

[RNase H2]

3.1.26.4

>5 U/μl

One unit is defined as the amount of enzyme that can cleave 1 nmol of synthetic DNA/RNA hybrid double-stranded body substrate containing a single R-loop per min at 37°C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Feng S, Cao Z. Is the role of human RNase H2 restricted to its enzyme activity? Prog Biophys Mol Biol. 2016 May;121(1):66-73.  [Content Brief]

  [2]. Chon H, et al. RNase H2 roles in genome integrity revealed by unlinking its activities. Nucleic Acids Res. 2013 Mar 1;41(5):3130-43.  [Content Brief]