Sodium-binding benzofuran isophthalate 

SBFI is a cell-impermeant, fluorescent Na⁺ indicator dye. SBFI is excited at 340 nm and the fluorophore emission is collected at 450 nm^[1]. SBFI selective for Na⁺ over K⁺ with K_d values of 20 and 120 mM for these ions, respectively. ^[2].

The SBFI dye for each sample was prepared by : ^[1]
1. dissolving the appropriate quantity of dye powder in 3 mL of dimethyl sulphoxide.
2. The five solutions were agitated for 5 minutes.
3. Each sample was then exposed to ultraviolet light with a Leitz Orthoplan microscope, and fluorescence levels recorded.
4. Ten readings in total were achieved for each solution, agitating between readings.
5. For each set of sample results the highest, lowest, and median values were plotted against dye concentration.
6. From these tests the optimum fluorescence values, with minimum variation of upper and lower limits, was established with 50 g of SBFI dye.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

838.81

C₄₄H₄₂N₂O₁₅

124549-08-2

O=C(C1=CC=C(C(C(O)=O)=C1)C2=CC3=CC(OC)=C(N4CCOCCOCCN(CCOCC4)C5=C(OC)C=C6C=C(C7=CC=C(C(O)=O)C=C7C(O)=O)OC6=C5)C=C3O2)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Leeves MA, The effect of mechanical deformation on the distribution of ions in fibroblasts. Am J Orthod Dentofacial Orthop. 1995 Jun;107(6):625-32.  [Content Brief]

  [2]. Minta A, Fluorescent indicators for cytosolic sodium. J Biol Chem. 1989 Nov 15;264(32):19449-57.  [Content Brief]