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Results for "

Northern blotting

" in MedChemExpress (MCE) Product Catalog:

3

Inhibitors & Agonists

1

Biochemical Assay Reagents

Cat. No. Product Name Target Research Areas Chemical Structure
  • HY-160841
    Biotin-16-dCTP

    		DNA Stain DNA/RNA Synthesis Others
    Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate that serves as an important DNA labeling substrate. Biotin-16-dCTP can be enzymatically incorporated into the 3' end of DNA probes via terminal deoxynucleotidyl transferase, forming a 1-3 nucleotide-long tail to achieve biotinylation of the probes. Biotin-16-dCTP enhances chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors through Northern blotting. Biotin-16-dCTP can also replace conventional dCTP to be integrated into single-stranded DNA generated by asymmetric polymerase chain reaction, which is applicable for bioconjugation or pull-down assays. Repeated freeze-thaw cycles of Biotin-16-dCTP should be avoided to prevent degradation of its function for probe biotinylation .
    Biotin-16-dCTP
  • HY-155743A
    ddUTP lithium, 100 mM in H2O

    2',3'-Dideoxyuridine-5'-triphosphate lithium

    		 Nucleoside Antimetabolite/Analog Others
    ddUTP (lithium), 100 mM in H2O is a nucleotide analog that can be incorporated into DNA, RNA, or other nucleic acids. ddUTP (lithium), 100 mM in H2O can be used to prepare DNA/RNA hybridization experiments, such as Southern blots and Northern blots.
    ddUTP lithium, 100 mM in H2O
  • HY-117921
    DA-E 5090

    		Interleukin Related Inflammation/Immunology
    DA-E 5090 is an orally effective inhibitor of IL-1 production that can be converted into a pharmacologically active deacetylated form (DA-E5090) in vivo. In this study, the effects of DA-E5090 on IL-1 production in vitro were examined by LPS-stimulated human monocytes. The results showed that DA-E5090 could dose-dependently inhibit the production of IL-1α and IL-1β (1-10 μM) by LPS-stimulated human monocytes, as determined by LAF assay and ELISA. Northern blotting analysis showed that DA-E5090 inhibited the transcription of IL-1α and IL-1β mRNA.
    DA-E 5090

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