Biotin-16-dCTP
Based on 1 Customer Validation
Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate that serves as an important DNA labeling substrate. Biotin-16-dCTP can be enzymatically incorporated into the 3' end of DNA probes via terminal deoxynucleotidyl transferase, forming a 1-3 nucleotide-long tail to achieve biotinylation of the probes. Biotin-16-dCTP enhances chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors through Northern blotting. Biotin-16-dCTP can also replace conventional dCTP to be integrated into single-stranded DNA generated by asymmetric polymerase chain reaction, which is applicable for bioconjugation or pull-down assays. Repeated freeze-thaw cycles of Biotin-16-dCTP should be avoided to prevent degradation of its function for probe biotinylation.
For research use only. We do not sell to patients.
- Purity : 92.72%
- CAS No.: 2567941-52-8
- Formula: C32H51N8O17P3S
- Molecular Weight:944.78
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Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
Description
In Vitro
Biotin-16-dCTP (0.1 mM; 60 min at 37 °C) enables enzymatic biotinylation of DNA probes to produce 1-3 nt biotin tails, which enhance the sensitivity of chemiluminescent Northern blotting for detecting specific tRNAs and tRNA-derived fragments from Shewanella glacialimarina and Saccharomyces cerevisiae[1].
Biotin-16-dCTP (0.01 mM; 60 min) successfully adds 1-3 biotin moieties to DNA oligonucleotide probes, generating high-signal probes suitable for chemiluminescent northern blot detection of specific tRNAs and tRNA-derived fragments from Shewanella glacialimarina TZS-4T and Saccharomyces cerevisiae BY4741[2].
Biotin-16-dCTP (up to 100% replacement of canonical dCTP) is incorporated into ssDNA synthesized via aPCR[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2567941-52-8
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Appearance Oil
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Molecular Weight 944.78
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Formula C32H51N8O17P3S
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Color White to off-white
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SMILES
O[C@H]1C[C@H](N2C(N=C(N)C(C#CCNC(CCCNC(CCCCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=O)=C2)=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
H2O : 5.88 mg/mL (6.22 mM; ultrasonic and warming and adjust pH to 12 with 1 M NaOH and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Pull-down
The pull-down assay is an in vitro technique used to detect physical interactions between two or more proteins and an invaluable tool for confirming a predicted protein-protein interaction or identifying novel interacting partners. This method typically involves the use of affinity purification with various wash and elution steps.
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Protocol for Northern Blot
Northern blot detects a defined RNA species by separating denatured RNA by size, transferring RNA to a membrane, hybridizing with a complementary labeled DNA or RNA probe, and detecting probe-bound RNA by autoradiography, phosphorimaging, or validated nonradioactive detection. The readout is both RNA size and abundance: band migration estimates transcript length or RNA-processing state, while band intensity reflects relative target RNA amount after normalization to total RNA, rRNA, or another validated loading control. In cancer cells, primary neurons, mouse tumor samples, intestinal organoids, inflammatory macrophages, or drug-screening studies, Northern blot is most appropriate when transcript size, isoform pattern, RNA processing, or small-RNA detection is important; qPCR or RNA-seq can complement it when higher sensitivity or global profiling is needed.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2]. Gregorova P, et al. Protocol for rapid tRNA enrichment and chemiluminescent northern blot detection of tRNA and tRNA-derived fragments. STAR Protoc. 2026;7(1):104357. [Content Brief]
[3]. Veneziano R, et al. In vitro synthesis of gene-length single-stranded DNA. Sci Rep. 2018;8(1):6548. Published 2018 Apr 25. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.0584 mL | 5.2922 mL | 10.5845 mL | 26.4612 mL |
| 5 mM | 0.2117 mL | 1.0584 mL | 2.1169 mL | 5.2922 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Biotin-16-dCTP Related Classifications
HY-160841 Related Classifications
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