2567941-52-8
Chemical Structure
Biotin-16-dCTP
- CAS No.: 2567941-52-8
- Formula:C32H51N8O17P3S
- Molecular Weight:944.78
InChIKey: ZASUZJWVLSBCNG-AYPTXYQFSA-N
SMILES: O[C@H]1C[C@H](N2C(N=C(N)C(C#CCNC(CCCNC(CCCCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=O)=C2)=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O
Biological Activity: Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate that serves as an important DNA labeling substrate. Biotin-16-dCTP can be enzymatically incorporated into the 3' end of DNA probes via terminal deoxynucleotidyl transferase, forming a 1-3 nucleotide-long tail to achieve biotinylation of the probes. Biotin-16-dCTP enhances chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors through Northern blotting. Biotin-16-dCTP can also replace conventional dCTP to be integrated into single-stranded DNA generated by asymmetric polymerase chain reaction, which is applicable for bioconjugation or pull-down assays. Repeated freeze-thaw cycles of Biotin-16-dCTP should be avoided to prevent degradation of its function for probe biotinylation[1][2][3].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Biotin-16-dCTP | 92.72% | Biotin-16-dCTP is a biotinylated deoxycytidine triphosphate that serves as an important DNA labeling substrate. Biotin-16-dCTP can be enzymatically incorporated into the 3' end of DNA probes via terminal deoxynucleotidyl transferase, forming a 1-3 nucleotide-long tail to achieve biotinylation of the probes. Biotin-16-dCTP enhances chemiluminescent detection of low-abundance targets such as specific tRNA isoacceptors through Northern blotting. Biotin-16-dCTP can also replace conventional dCTP to be integrated into single-stranded DNA generated by asymmetric polymerase chain reaction, which is applicable for bioconjugation or pull-down assays. Repeated freeze-thaw cycles of Biotin-16-dCTP should be avoided to prevent degradation of its function for probe biotinylation. | ||||||||||||||||||||
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References
- [1]. Gregorova P, et al. Rapid tRNA Isolation and Chemiluminescent Northern Blot Detection of tRNA and tRNA-Derived Fragments[J]. bioRxiv, 2025: 2025.10. 14.681074.
- [2]. Gregorova P, et al. Protocol for rapid tRNA enrichment and chemiluminescent northern blot detection of tRNA and tRNA-derived fragments. STAR Protoc. 2026;7(1):104357. [Content Brief]
- [3]. Veneziano R, et al. In vitro synthesis of gene-length single-stranded DNA. Sci Rep. 2018;8(1):6548. Published 2018 Apr 25. [Content Brief]
