1. Cytoskeleton
  2. Gap Junction Protein

Tonabersat (Synonyms: SB-220453)

Cat. No.: HY-15204 Purity: 98.48%
Handling Instructions

Tonabersat is a gap-junction modulator.

For research use only. We do not sell to patients.
Tonabersat Chemical Structure

Tonabersat Chemical Structure

CAS No. : 175013-84-0

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 224 In-stock
5 mg USD 204 In-stock
10 mg USD 343 In-stock
50 mg USD 862 In-stock
100 mg   Get quote  
200 mg   Get quote  

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    Tonabersat purchased from MCE. Usage Cited in: Patent. US 20170258758 A1.

    ELISA of IFNα and TNFα in conditioned media from co-cultured MDA231-BrM2 cell and astrocytes in the presence of Tonabersat (Tona) or meclofenamate (Meclo) with indicated concentrations.

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    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Tonabersat is a gap-junction modulator.

    IC50 & Target

    Gap-junction[1]

    In Vitro

    Tonabersat, a novel benzopyran derivative, inhibits cortical spreading depression (CSD) and therefore might be able to inhibit the early migraine mechanisms[1].

    In Vivo

    Treatment with either Tonabersat (10 mg/kg) or Meclofenamate (20 mg/kg) as single agents significantly inhibit progression of metastatic lesions. Addition of carboplatin to either agent profoundly inhibits brain metastasis[2].

    Clinical Trial
    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 2.5522 mL 12.7610 mL 25.5219 mL
    5 mM 0.5104 mL 2.5522 mL 5.1044 mL
    10 mM 0.2552 mL 1.2761 mL 2.5522 mL
    Please refer to the solubility information to select the appropriate solvent.
    Animal Administration
    [2]

    Tonabersat (purphased from MedChem Express) is prepared in 10% DMSO in polyethylene glycol 400 (Mice)[2].

    Mice[2]
    Athymic NCR nu/nu mice, Cr:NIH bg-nu-xid mice, B6129SF1/J, C57BL/6J-Tmem173gt/J ‘golden ticket’, and C57/Bl/6J mice are used at 5-6 weeks of age. For inducible knockdown experiments, mice are given doxycycline hyclate in the drinking water (2 mg/mL) and the diet 14 days after injection of cancer cells. For drug treatment experiments, mice are intraperitoneally injected with Carboplatin (5 mg/kg per 5 days), Tonabersat (MedChem Express) (10 mg/kg per day), or meclofenamic acid sodium salt (20 mg/kg per day). Vehicle (10% DMSO in polyethylene glycol 400) is used in control mice. Quantification of tumour burden is by Bio-luminescent imaging (BLI), performed using an IVIS Spectrum Xenogen instrument and analysed using Living Image software v.2.50. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    391.82

    Formula

    C₂₀H₁₉ClFNO₄

    CAS No.

    175013-84-0

    SMILES

    O[[email protected]@H]1C(C)(C)OC2=CC=C(C(C)=O)C=C2[[email protected]@H]1NC(C3=CC(Cl)=C(F)C=C3)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    10 mM in DMSO

    Tonabersat is prepared in vehicle (10% DMSO in PEG 400)[2].
    Tonabersat is dissolved in 60% cyclodextrin and 40% PEG[3].

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    References

    Purity: 98.48%

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    Product Name:
    Tonabersat
    Cat. No.:
    HY-15204
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